Universität zu Köln

Tsirigoti, Amerssa, Tzovenis, I, Koutsaviti, A., Economou-Amilli, A., Loannou, E. and Melkonian, M. (2020). Biofilm cultivation of marine dinoflagellates under different temperatures and nitrogen regimes enhances DHA productivity. J. Appl. Phycol., 32 (2). S. 865 - 881. DORDRECHT: SPRINGER. ISSN 1573-5176

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Abstract

Dinoflagellates contain large amounts of omega-3 fatty acids, including the nutritionally important docosahexaenoic acid (DHA). However, their cultivation in suspensions is characterized by low growth rates. Twin-layer porous substrate photobioreactors (TL-PSBRs) have been shown to support growth of different microalgal species, including the robust dinoflagellate Symbiodinium voratum. In the present study, the potential of cultivating marine autotrophic dinoflagellate species in a TL-PSBR for DHA production was explored. Based on initial screening experiments, two Symbiodinium species with high biomass and DHA productivities were selected: the symbiotic Symbiodinium microadriaticum CCAC 2475 B and the free-living Symbiodinium voratum CCAC 3869 B. The effects of three different temperatures (17, 22 and 27 degrees C) and nitrogen regimes (nitrate, ammonium and nitrogen-free) on biomass growth, total lipid accumulation and fatty acid methyl esters (FAMEs) content, with emphasis on DHA, were evaluated. The two lower temperatures (17 and 22 degrees C) enhanced growth and total lipid accumulation of S. microadriaticum CCAC 2475 B and S. voratum CCAC 3869 B. Cultivation at 22 degrees C and nitrogen limitation led to a significant positive effect on DHA productivity. Symbiodinium. microadriaticum CCAC 2475 B reached a DHA productivity of 145.4 mg m(-2) day(-1) and DHA content in the dry biomass of 2% (w/w) after 4 days of nitrogen depletion. The results of the present study demonstrated that autotrophic dinoflagellates, when cultivated on a TL-PSBR, produce comparable amounts of lipids and fatty acids to other commercially used microalgal species including the valuable DHA.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Tsirigoti, Amerssa UNSPECIFIED UNSPECIFIED UNSPECIFIED Tzovenis, I UNSPECIFIED UNSPECIFIED UNSPECIFIED Koutsaviti, A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Economou-Amilli, A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Loannou, E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Melkonian, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-338318
DOI:	10.1007/s10811-019-02027-w
Journal or Publication Title:	J. Appl. Phycol.
Volume:	32
Number:	2
Page Range:	S. 865 - 881
Date:	2020
Publisher:	SPRINGER
Place of Publication:	DORDRECHT
ISSN:	1573-5176
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language FATTY-ACID-COMPOSITION; FISH-OIL; LIPID-CONTENT; GROWTH-PHASE; MICROALGAE; CULTURE; FOOD; SYMBIODINIUM; BIODIESEL; EPA Multiple languages Biotechnology & Applied Microbiology; Marine & Freshwater Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/33831