Universität zu Köln

Comelli, Petra, Glowa, Dorothea, Frerichs, Anneke, Engelhorn, Julia, Chandler, John W. and Werr, Wolfgang (2020). Functional dissection of the DORNRoSCHEN-LIKE enhancer 2 during embryonic and phyllotactic patterning. Planta, 251 (4). NEW YORK: SPRINGER. ISSN 1432-2048

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Abstract

Main conclusion The Arabidopsis DORNRoSCHEN-LIKE enhancer 2 comprises a high-occupancy target region in the IM periphery that integrates signals for the spiral phyllotactic pattern and cruciferous arrangement of sepals. Transcription of the DORNRoSCHEN-LIKE (DRNL) gene marks lateral organ founder cells (LOFCs) in the peripheral zone of the inflorescence meristem (IM) and enhancer 2 (En2) in the DRNL promoter upstream region essentially contributes to this phyllotactic transcription pattern. Further analysis focused on the phylogenetically highly conserved 100-bp En2(core) element, which was sufficient to promote the phyllotactic pattern, but was recalcitrant to further shortening. Here, we show that En2(core) functions independent of orientation and create a series of mutations to study consequences on the transcription pattern. Their analysis shows that, first, in addition to in the inflorescence apex, En2(core) acts in the embryo; second, cis-regulatory target sequences are distributed throughout the 100-bp element, although substantial differences exist in their function between embryo and IM. Third, putative core auxin response elements (AuxREs) spatially activate or restrict DRNL expression, and fourth, according to chromatin configuration data, En2(core) enhancer activity in LOFCs correlates with an open chromatin structure at the DRNL transcription start. In combination, mutational and chromatin analyses imply that En2(core) comprises a high-occupancy target (HOT) region for transcription factors, which implements phyllotactic information for the spiral LOFC pattern in the IM periphery and coordinates the cruciferous array of floral sepals. Our data disfavor a contribution of activating auxin response factors (ARFs) but do not exclude auxin as a morphogenetic signal.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Comelli, Petra UNSPECIFIED UNSPECIFIED UNSPECIFIED Glowa, Dorothea UNSPECIFIED UNSPECIFIED UNSPECIFIED Frerichs, Anneke UNSPECIFIED UNSPECIFIED UNSPECIFIED Engelhorn, Julia UNSPECIFIED UNSPECIFIED UNSPECIFIED Chandler, John W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Werr, Wolfgang UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-339926
DOI:	10.1007/s00425-020-03381-7
Journal or Publication Title:	Planta
Volume:	251
Number:	4
Date:	2020
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1432-2048
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ARABIDOPSIS GENE MONOPTEROS; AUXIN-RESPONSE; DORNRA-SCHEN; TRANSCRIPTION; INITIATION; EXPRESSION; THALIANA Multiple languages Plant Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/33992