Gebauer, Jan M., Flachsenberg, Florian ORCID: 0000-0001-7051-8719, Windler, Cordula, Richer, Barbara, Baumann, Ulrich and Seeger, Karsten (2020). Structural and biophysical characterization of the type VII collagen vWFA2 subdomain leads to identification of two binding sites. FEBS Open Bio, 10 (4). S. 580 - 593. HOBOKEN: WILEY. ISSN 2211-5463

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Abstract

Type VII collagen is an extracellular matrix protein, which is important for skin stability; however, detailed information at the molecular level is scarce. The second vWFA (von Willebrand factor type A) domain of type VII collagen mediates important interactions, and immunization of mice induces skin blistering in certain strains. To understand vWFA2 function and the pathophysiological mechanisms leading to skin blistering, we structurally characterized this domain by X-ray crystallography and NMR spectroscopy. Cell adhesion assays identified two new interactions: one with beta 1 integrin via its RGD motif and one with laminin-332. The latter interaction was confirmed by surface plasmon resonance with a K-D of about 1 mm. These data show that vWFA2 has additional functions in the extracellular matrix besides interacting with type I collagen.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Gebauer, Jan M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Flachsenberg, FlorianUNSPECIFIEDorcid.org/0000-0001-7051-8719UNSPECIFIED
Windler, CordulaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Richer, BarbaraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Baumann, UlrichUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Seeger, KarstenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-340979
DOI: 10.1002/2211-5463.12807
Journal or Publication Title: FEBS Open Bio
Volume: 10
Number: 4
Page Range: S. 580 - 593
Date: 2020
Publisher: WILEY
Place of Publication: HOBOKEN
ISSN: 2211-5463
Language: English
Faculty: Faculty of Mathematics and Natural Sciences
Divisions: Faculty of Mathematics and Natural Sciences > Department of Chemistry > Institute of Biochemistry
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
EPIDERMOLYSIS-BULLOSA ACQUISITA; PROTEIN SECONDARY STRUCTURE; NC1 DOMAIN; NMR; ALIGNMENT; MODEL; PREDICTION; LAMININ-5; FIBRONECTIN; REGIONMultiple languages
Biochemistry & Molecular BiologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/34097

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