Niether, Doreen ORCID: 0000-0002-8496-4215, Sarter, Mona ORCID: 0000-0003-1867-5543, Koenig, Bernd W., Fitter, Joerg ORCID: 0000-0002-4503-2079, Stadler, Andreas M. and Wiegand, Simone ORCID: 0000-0001-6333-1956 (2020). Thermophoresis: The Case of Streptavidin and Biotin. Polymers, 12 (2). BASEL: MDPI. ISSN 2073-4360

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Abstract

Thermophoretic behavior of a free protein changes upon ligand binding and gives access to information on the binding constants. The Soret effect has also been proven to be a promising tool to gain information on the hydration layer, as the temperature dependence of the thermodiffusion behavior is sensitive to solute-solvent interactions. In this work, we perform systematic thermophoretic measurements of the protein streptavidin (STV) and of the complex STV with biotin (B) using thermal diffusion forced Rayleigh scattering (TDFRS). Our experiments show that the temperature sensitivity of the Soret coefficient is reduced for the complex compared to the free protein. We discuss our data in comparison with recent quasi-elastic neutron scattering (QENS) measurements. As the QENS measurement has been performed in heavy water, we perform additional measurements in water/heavy water mixtures. Finally, we also elucidate the challenges arising from the quantiative thermophoretic study of complex multicomponent systems such as protein solutions.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Niether, DoreenUNSPECIFIEDorcid.org/0000-0002-8496-4215UNSPECIFIED
Sarter, MonaUNSPECIFIEDorcid.org/0000-0003-1867-5543UNSPECIFIED
Koenig, Bernd W.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Fitter, JoergUNSPECIFIEDorcid.org/0000-0002-4503-2079UNSPECIFIED
Stadler, Andreas M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wiegand, SimoneUNSPECIFIEDorcid.org/0000-0001-6333-1956UNSPECIFIED
URN: urn:nbn:de:hbz:38-346201
DOI: 10.3390/polym12020376
Journal or Publication Title: Polymers
Volume: 12
Number: 2
Date: 2020
Publisher: MDPI
Place of Publication: BASEL
ISSN: 2073-4360
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
THERMAL-DIFFUSION; SORET COEFFICIENT; REFRACTIVE-INDEX; HEAVY-WATER; MIXTURES; ACCUMULATION; DEPENDENCE; BEHAVIOR; POLYMER; BINDINGMultiple languages
Polymer ScienceMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/34620

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