Universität zu Köln

Fam, Nevine S., Gamal, Doaa, Mohamed, Sara H., Wasfy, Reham M., Soliman, May S., El-Kholy, Amani A. and Higgins, Paul G. (2020). Molecular Characterization of Carbapenem/Colistin-Resistant Acinetobacter baumannii Clinical Isolates from Egypt by Whole-Genome Sequencing. Infect. Drug Resistance, 13. S. 4487 - 4494. ALBANY: DOVE MEDICAL PRESS LTD. ISSN 1178-6973

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Abstract

Purpose: The rise of carbapenem-resistant A. baumannii (CRAB) is considered a public health problem limiting the treatment options. Our current work studied the emergence and mechanisms of colistin-resistance among CRAB isolates in Egypt. Materials and Methods: Seventeen clinically recovered A. baumannii were identified and screened for their antimicrobial susceptibilities using VITEK-2 system. Colistin susceptibility was evaluated using broth microdilution, and characterization of carbapenem/colistin resistance determinants was performed using whole-genome sequencing (Illumina MiSeq). Results: About 52.9% (9/17) were colistin-resistant. PCR results revealed that all isolates carried bla(OXA-51-like genes), bla(OXA-23-like) was detected in 82.3% (14/17) and bla(NDM) in 23.5% (4/17). Two isolates harboured bla(GES-35) and bla(OXA-23). Furthermore, genome analysis of seven isolates revealed six belonged to international clone 2 (IC2) while the remaining isolate was a singleton (ST158), representing a clone circulating in Mediterranean/Middle Eastern countries. Conclusion: The emergence and high incidence of colistin-resistance among CRAB clinical isolates in Egypt are alarming because it further limits therapy options and requires prudent antimicrobial stewardship and stringent infection control measures. Whole-genome sequence analyses suggest that the resistance to colistin was associated with multiple mutations in the pmrCAB genes. The high incidence of the high-risk lineage IC2 harbouring bla(OXA-23-like) as well as bla(NDM) is also of concern.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Fam, Nevine S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Gamal, Doaa UNSPECIFIED UNSPECIFIED UNSPECIFIED Mohamed, Sara H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Wasfy, Reham M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Soliman, May S. UNSPECIFIED UNSPECIFIED UNSPECIFIED El-Kholy, Amani A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Higgins, Paul G. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-349437
DOI:	10.2147/IDR.S288865
Journal or Publication Title:	Infect. Drug Resistance
Volume:	13
Page Range:	S. 4487 - 4494
Date:	2020
Publisher:	DOVE MEDICAL PRESS LTD
Place of Publication:	ALBANY
ISSN:	1178-6973
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language COLISTIN RESISTANCE; POLYMYXIN-B Multiple languages Infectious Diseases; Pharmacology & Pharmacy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/34943