Universität zu Köln

Kern, Ramona, Facchinelli, Fabio, Delwiche, Charles ORCID: 0000-0001-7854-8584, Weber, Andreas P. M., Bauwe, Hermann and Hagemann, Martin (2020). Evolution of Photorespiratory Glycolate Oxidase among Archaeplastida. Plants-Basel, 9 (1). BASEL: MDPI. ISSN 2223-7747

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Abstract

Photorespiration has been shown to be essential for all oxygenic phototrophs in the present-day oxygen-containing atmosphere. The strong similarity of the photorespiratory cycle in cyanobacteria and plants led to the hypothesis that oxygenic photosynthesis and photorespiration co-evolved in cyanobacteria, and then entered the eukaryotic algal lineages up to land plants via endosymbiosis. However, the evolutionary origin of the photorespiratory enzyme glycolate oxidase (GOX) is controversial, which challenges the common origin hypothesis. Here, we tested this hypothesis using phylogenetic and biochemical approaches with broad taxon sampling. Phylogenetic analysis supported the view that a cyanobacterial GOX-like protein of the 2-hydroxy-acid oxidase family most likely served as an ancestor for GOX in all eukaryotes. Furthermore, our results strongly indicate that GOX was recruited to the photorespiratory metabolism at the origin of Archaeplastida, because we verified that Glaucophyta, Rhodophyta, and Streptophyta all express GOX enzymes with preference for the substrate glycolate. Moreover, an ancestral protein synthetically derived from the node separating all prokaryotic from eukaryotic GOX-like proteins also preferred glycolate over l-lactate. These results support the notion that a cyanobacterial ancestral protein laid the foundation for the evolution of photorespiratory GOX enzymes in modern eukaryotic phototrophs.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kern, Ramona UNSPECIFIED UNSPECIFIED UNSPECIFIED Facchinelli, Fabio UNSPECIFIED UNSPECIFIED UNSPECIFIED Delwiche, Charles UNSPECIFIED orcid.org/0000-0001-7854-8584 UNSPECIFIED Weber, Andreas P. M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Bauwe, Hermann UNSPECIFIED UNSPECIFIED UNSPECIFIED Hagemann, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-351444
DOI:	10.3390/plants9010106
Journal or Publication Title:	Plants-Basel
Volume:	9
Number:	1
Date:	2020
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2223-7747
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language L-LACTATE OXIDASE; PHYLOGENETIC ANALYSIS; CYANOPHORA-PARADOXA; ORIGIN; ACID; ENZYMES; PHOTOSYNTHESIS; DEHYDROGENASE; CYANOBACTERIA; PEROXISOMES Multiple languages Plant Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/35144