Universität zu Köln

Mizi, Athanasia ORCID: 0000-0002-4313-491X, Gusmao, Eduardo Gade and Papantonis, Argyris ORCID: 0000-0001-7551-1073 (2020). iHi-C 2.0: A simple approach for mapping native spatial chromatin organisation from low cell numbers. Methods, 170. S. 33 - 38. SAN DIEGO: ACADEMIC PRESS INC ELSEVIER SCIENCE. ISSN 1095-9130

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Abstract

Genome organization is now understood to be tightly linked to all genomic functions. Thus, the high-resolution mapping of higher-order chromosomal structures via 3C-based approaches has become an integral tool for studying transcriptional and cell cycle regulation, signaling effects or disease onset. Nonetheless, 3C-based protocols are not without caveats, like dependencies on fixation conditions, restriction enzyme pervasiveness in crosslinked chromatin and ligation efficiency. To address some of these caveats, we describe here the streamlined iHi-C 2.0 protocol that allows for the genome-wide interrogation of native spatial chromatin contacts without a need for chemical fixation. This approach improves ligation efficiency and presents minimal material losses, and is thus suitable for analysing samples with limiting cell numbers. Following high throughput sequencing, iHi-C 2.0 generates high signal-to-noise and focal maps of the interactions within and between mammalian chromosomes under native conditions.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Mizi, Athanasia UNSPECIFIED orcid.org/0000-0002-4313-491X UNSPECIFIED Gusmao, Eduardo Gade UNSPECIFIED UNSPECIFIED UNSPECIFIED Papantonis, Argyris UNSPECIFIED orcid.org/0000-0001-7551-1073 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-352016
DOI:	10.1016/j.ymeth.2019.07.003
Journal or Publication Title:	Methods
Volume:	170
Page Range:	S. 33 - 38
Date:	2020
Publisher:	ACADEMIC PRESS INC ELSEVIER SCIENCE
Place of Publication:	SAN DIEGO
ISSN:	1095-9130
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HI-C; GENOME; TRANSCRIPTION; CONFORMATION; REVEALS; CAPTURE Multiple languages Biochemical Research Methods; Biochemistry & Molecular Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/35201