Universität zu Köln

Bruns, Michael, Wanger, Jara, Utermoehlen, Olaf and Deppert, Wolfgang (2015). An inducible transgenic mouse breast cancer model for the analysis of tumor antigen specific CD8(+) T-cell responses. Oncotarget, 6 (36). S. 38487 - 38504. ORCHARD PARK: IMPACT JOURNALS LLC. ISSN 1949-2553

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Abstract

In Simian virus 40 (SV40) transgenic BALB/c WAP-T mice tumor development and progression is driven by SV40 tumor antigens encoded by inducible transgenes. WAP-T mice constitute a well characterized mouse model for breast cancer with strong similarities to the corresponding human disease. BALB/c mice mount only a weak cellular immune response against SV40 T-antigen (T-Ag). For studying tumor antigen specific CD8(+) T-cell responses against transgene expressing cells, we created WAP-T-NP mice, in which the transgene additionally codes for the NP118-126-epitope contained within the nucleoprotein of lymphocytic choriomeningitis virus (LCMV), the immune-dominant T-cell epitope in BALB/c mice. We then investigated in WAP-T-NP mice the immune responses against SV40 tumor antigens and the NP-epitope within the chimeric T-Ag/ NP protein (T-AgNP). Analysis of the immune-reactivity against T-Ag in WAP-T and of T-AgNP in WAP-T-NP mice revealed that, in contrast to wild type (wt) BALB/c mice, WAP-T and WAP-T-NP mice were non-reactive against T-Ag. However, like wtBALB/c mice, WAP-T as well as WAP-T-NP mice were highly reactive against the immune-dominant LCMV NP-epitope, thereby allowing the analysis of NP-epitope specific cellular immune responses in WAP-T-NP mice. LCMV infection of WAP-T-NP mice induced a strong, LCMV NP-epitope specific CD8+ T-cell response, which was able to specifically eliminate T-AgNP expressing mammary epithelial cells both prior to tumor formation (i.e. in cells of lactating mammary glands), as well as in invasive tumors. Elimination of tumor cells, however, was only transient, even after repeated LCMV infections. Further studies showed that already non-infected WAP-T-NP tumor mice contained LCMV NP-epitope specific CD8+ T-cells, albeit with strongly reduced, though measurable activity. Functional impairment of these 'endogenous' NP-epitope specific T-cells seems to be caused by expression of the programmed death-1 protein (PD1), as anti-PD1 treatment of splenocytes from WAP-T-NP tumor mice restored their activity. These characteristics are similar to those found in many tumor patients and render WAP-T-NP mice a suitable model for analyzing parameters to overcome the blockade of immune checkpoints in tumor patients.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Bruns, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Wanger, Jara UNSPECIFIED UNSPECIFIED UNSPECIFIED Utermoehlen, Olaf UNSPECIFIED UNSPECIFIED UNSPECIFIED Deppert, Wolfgang UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-386911
DOI:	10.18632/oncotarget.5750
Journal or Publication Title:	Oncotarget
Volume:	6
Number:	36
Page Range:	S. 38487 - 38504
Date:	2015
Publisher:	IMPACT JOURNALS LLC
Place of Publication:	ORCHARD PARK
ISSN:	1949-2553
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language LYMPHOCYTIC CHORIOMENINGITIS VIRUS; SIMIAN-VIRUS-40; IMMUNIZATION; MICE; IDENTIFICATION; INHIBITION; INFECTION; PROTEINS Multiple languages Oncology; Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/38691