Universität zu Köln

Kotz, Sandra, Kullmann, Maximilian, Crone, Barbara, Kalayda, Ganna V., Jaehde, Ulrich and Metzger, Sabine (2015). Combination of two-dimensional gel electrophoresis and a fluorescent carboxyfluorescein-diacetate-labeled cisplatin analogue allows the identification of intracellular cisplatin-protein adducts. Electrophoresis, 36 (21). S. 2811 - 2820. HOBOKEN: WILEY. ISSN 1522-2683

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Abstract

Cisplatin is one of the most widely used anticancer agents, but a major problem for successful chemotherapy is the development of drug resistance of tumor cells against cisplatin. Resistance to cisplatin is a multifactorial problem. A method to detect and identify intracellular cisplatin-protein adducts was developed using a fluorescent carboxyfluoresceindiacetate-labeled cisplatin analogue (CFDA-cisplatin), 2DE, and ESI-MS/MS. We identified several CFDA-cisplatin-protein adducts including members of the protein disulfide isomerase family (PDI). These are the first results of the detection of intracellular CFDA-cisplatin-protein adducts, which may help to understand the resistance mechanism of cisplatin.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kotz, Sandra UNSPECIFIED UNSPECIFIED UNSPECIFIED Kullmann, Maximilian UNSPECIFIED UNSPECIFIED UNSPECIFIED Crone, Barbara UNSPECIFIED UNSPECIFIED UNSPECIFIED Kalayda, Ganna V. UNSPECIFIED UNSPECIFIED UNSPECIFIED Jaehde, Ulrich UNSPECIFIED UNSPECIFIED UNSPECIFIED Metzger, Sabine UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-387799
DOI:	10.1002/elps.201500188
Journal or Publication Title:	Electrophoresis
Volume:	36
Number:	21
Page Range:	S. 2811 - 2820
Date:	2015
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1522-2683
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DISULFIDE-ISOMERASE; ENDOPLASMIC-RETICULUM; PLATINUM COMPLEXES; BINDING PROTEINS; CELL-DEATH; ICP-MS; RESISTANCE; MECHANISMS; CYS(374); THERAPY Multiple languages Biochemical Research Methods; Chemistry, Analytical Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/38779