Universität zu Köln

Siapich, Siarhei A., Akhtar, Isha, Hescheler, Jurgen, Schneider, Toni and Lueke, Matthias (2015). Low concentrations of ethanol but not of dimethyl sulfoxide (DMSO) impair reciprocal retinal signal transduction. Graefes Arch. Clin. Exp. Ophthalmol., 253 (10). S. 1713 - 1720. NEW YORK: SPRINGER. ISSN 1435-702X

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Abstract

The model of the isolated and superfused retina provides the opportunity to test drugs and toxins. Some chemicals have to be applied using low concentrations of organic solvents as carriers. Recently, E-/R-type (Ca(v)2.3) and T-type (Ca(v)3.2) voltage-gated Ca2+ channels were identified as participating in reciprocal inhibitory retinal signaling. Their participation is apparent, when low concentrations of NiCl2 (15 mu M) are applied during superfusion leading to an increase of the ERG b-wave amplitude, which is explained by a reduction of amacrine GABA-release onto bipolar neurons. During these investigations, differences were observed for the solvent carrier used. Recording of the transretinal receptor potentials from the isolated bovine retina. The pretreatment of bovine retina with 0.01 % (v/v) dimethylsulfoxide did not impair the NiCl2-mediated increase of the b-wave amplitude, which was 1.31-fold +/- 0.03 of initial value (n = 4). However, pretreatment of the retina with the same concentration of ethanol impaired reciprocal signaling (0.96-fold +/- 0.05, n = 4). Further, the implicit time of the b-wave was increased, suggesting that ethanol itself but not DMSO may antagonize GABA-receptors. Ethanol itself but not DMSO may block GABA receptors and cause an amplitude increase by itself, so that reciprocal signaling is impaired.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Siapich, Siarhei A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Akhtar, Isha UNSPECIFIED UNSPECIFIED UNSPECIFIED Hescheler, Jurgen UNSPECIFIED UNSPECIFIED UNSPECIFIED Schneider, Toni UNSPECIFIED UNSPECIFIED UNSPECIFIED Lueke, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-391765
DOI:	10.1007/s00417-015-3070-7
Journal or Publication Title:	Graefes Arch. Clin. Exp. Ophthalmol.
Volume:	253
Number:	10
Page Range:	S. 1713 - 1720
Date:	2015
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1435-702X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CALCIUM-CHANNEL SUBUNITS; ROD BIPOLAR CELLS; RAT RETINA; MURINE RETINA; BOVINE RETINA; RESPONSES; ELECTRORETINOGRAM; STIMULATION; ANTAGONISTS; INHIBITION Multiple languages Ophthalmology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/39176