Universität zu Köln

Refaian, Nasrin, Schlereth, Simona L., Koch, Konrad R., Notara, Maria, Hos, Deniz, Mescher, Melina, Iden, Sandra ORCID: 0000-0003-2333-9827, Bosch, Jacobus J., Jager, Martine J., Cursiefen, Claus and Heindl, Ludwig M. (2015). Comparing the Hem- and Lymphangiogenic Profile of Conjunctival and Uveal Melanoma Cell Lines. Invest. Ophthalmol. Vis. Sci., 56 (9). S. 5691 - 5698. ROCKVILLE: ASSOC RESEARCH VISION OPHTHALMOLOGY INC. ISSN 1552-5783

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Abstract

PURPOSE. Malignant melanomas of the ocular surface (conjunctival melanoma [CM]) and within the eye (uveal melanoma [UM]) show different types of metastatic behavior. While CM has a propensity to spread first to regional lymph nodes, UM metastasizes almost exclusively via the hematogenic route to the liver. We investigated whether these different metastatic patterns might be attributable to differential hem-and lymphangiogenic characteristics of CM and UM cells. METHODS. Human CM (CM2005.1, CRMM1, CRMM2) and UM (Mel270, Mel290, OM431) cell lines were analyzed for VEGF-A, -C, and -D expression by RT-PCR and ELISA. The influence of CM-or UM-conditioned medium on blood (BEC) and lymphatic (LEC) endothelial cell proliferation and migration was measured using 3-[4,5-dimethylthiazol-2yl]-2,5-diphenyltetrazolium bromide (MTT) and scratch assays, respectively. RESULTS. Vascular endothelial growth factor-A, -C and -D mRNA, and VEGF-A and -D protein were expressed by all CM and UM cell lines, while VEGF-C protein was only expressed by UM cell lines. The CM-and UM-conditioned medium did neither differentially affect BEC (P = 0.86) and LEC (P = 0.90) proliferation, nor BEC (P = 0.56) and LEC (P = 0.90) migration. CONCLUSIONS. Conjunctival melanoma cell lines did not show a higher prolymphangiogenic potential, and UM cell lines did not show a higher prohemangiogenic potential. Accordingly, other mechanisms within the tumor microenvironment might account for the diverging metastatic patterns of conjunctival versus uveal melanomas.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Refaian, Nasrin UNSPECIFIED UNSPECIFIED UNSPECIFIED Schlereth, Simona L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Koch, Konrad R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Notara, Maria UNSPECIFIED UNSPECIFIED UNSPECIFIED Hos, Deniz UNSPECIFIED UNSPECIFIED UNSPECIFIED Mescher, Melina UNSPECIFIED UNSPECIFIED UNSPECIFIED Iden, Sandra UNSPECIFIED orcid.org/0000-0003-2333-9827 UNSPECIFIED Bosch, Jacobus J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Jager, Martine J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Cursiefen, Claus UNSPECIFIED UNSPECIFIED UNSPECIFIED Heindl, Ludwig M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-396836
DOI:	10.1167/iovs.15-16829
Journal or Publication Title:	Invest. Ophthalmol. Vis. Sci.
Volume:	56
Number:	9
Page Range:	S. 5691 - 5698
Date:	2015
Publisher:	ASSOC RESEARCH VISION OPHTHALMOLOGY INC
Place of Publication:	ROCKVILLE
ISSN:	1552-5783
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ENDOTHELIAL-GROWTH-FACTOR; TUMOR-ASSOCIATED LYMPHANGIOGENESIS; AMERICAN JOINT COMMITTEE; CILIARY-BODY-MELANOMAS; MALIGNANT MELANOMAS; FACTOR-A; VEGF-A; CANCER; ANGIOGENESIS; EXPRESSION Multiple languages Ophthalmology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/39683