Universität zu Köln

Xin, Wei, Heilig, Juliane, Paulsson, Mats and Zaucke, Frank (2015). Collagen II regulates chondroycte integrin expression profile and differentiation. Connect. Tissue Res., 56 (4). S. 307 - 315. LONDON: INFORMA HEALTHCARE. ISSN 1607-8438

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Abstract

Collagen II is the major fibril-forming collagen in cartilage. Complete absence of collagen II in mice is not compatible with life and in humans mutations in the COL2A1 gene lead to osteochondrodysplasias with diverse phenotypes. However, mechanistic studies on how chondrocytes respond to a lack of collagen II in their extracellular matrix are limited. Primary mouse chondrocytes were isolated from knee joints of newborn mice and transfected with siRNA targeting Col2 alpha(1) to suppress collagen II expression. The expression of integrin receptors and matrix proteins was investigated by RT-PCR and immunoblots. The localization of matrix components was evaluated by immunostaining. Signaling pathways and the differentiation state of chondrocytes was monitored by RT-PCR and flow cytometry. We demonstrate that in the absence of collagen II chondrocytes start to produce collagen I. Some binding partners of collagen II are partially lost from the matrix while other proteins, e.g. COMP, were still found associated with the newly formed collagen network. The lack of collagen II induced changes in the expression profile of integrins. Further, we detected alterations in the Indian hedgehog/parathyroid hormone-related protein (lhh/PTHrP) pathway that were accompanied by changes in the differentiation state of chondrocytes. Collagen II seems not to be essential for chondrocyte survival in culture but it plays an important role in maintaining chondrocyte differentiation. We suggest that a crosstalk between extracellular matrix and cells via integrins and the Ihh/PTHrP pathway is involved in regulating the differentiation state of chondrocytes.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Xin, Wei UNSPECIFIED UNSPECIFIED UNSPECIFIED Heilig, Juliane UNSPECIFIED UNSPECIFIED UNSPECIFIED Paulsson, Mats UNSPECIFIED UNSPECIFIED UNSPECIFIED Zaucke, Frank UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-397514
DOI:	10.3109/03008207.2015.1026965
Journal or Publication Title:	Connect. Tissue Res.
Volume:	56
Number:	4
Page Range:	S. 307 - 315
Date:	2015
Publisher:	INFORMA HEALTHCARE
Place of Publication:	LONDON
ISSN:	1607-8438
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language OLIGOMERIC MATRIX PROTEIN; EXTRACELLULAR-MATRIX; TRANSGENIC MICE; INDIAN HEDGEHOG; GROWTH-PLATE; CARTILAGE; CHONDROCYTES; MATRILIN-3; BONE; PHENOTYPE Multiple languages Cell Biology; Orthopedics Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/39751