Petersen, Hendrik O., Hoeger, Stefanie K., Looso, Mario ORCID: 0000-0003-1495-9530, Lengfeld, Tobias, Kuhn, Anne, Warnken, Uwe, Nishimiya-Fujisawa, Chiemi, Schnoelzer, Martina, Krueger, Marcus, Oezbek, Suat, Simakov, Oleg ORCID: 0000-0002-3585-4511 and Holstein, Thomas W. (2015). A Comprehensive Transcriptomic and Proteomic Analysis of Hydra Head Regeneration. Mol. Biol. Evol., 32 (8). S. 1928 - 1948. OXFORD: OXFORD UNIV PRESS. ISSN 1537-1719

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Abstract

The cnidarian freshwater polyp Hydra sp. exhibits an unparalleled regeneration capacity in the animal kingdom. Using an integrative transcriptomic and stable isotope labeling by amino acids in cell culture proteomic/phosphoproteomic approach, we studied stem cell-based regeneration in Hydra polyps. As major contributors to head regeneration, we identified diverse signaling pathways adopted for the regeneration response as well as enriched novel genes. Our global analysis reveals two distinct molecular cascades: an early injury response and a subsequent, signaling driven patterning of the regenerating tissue. A key factor of the initial injury response is a general stabilization of proteins and a net upregulation of transcripts, which is followed by a subsequent activation cascade of signaling molecules including Wnts and transforming growth factor (TGF) beta-related factors. We observed moderate overlap between the factors contributing to proteomic and transcriptomic responses suggesting a decoupled regulation between the transcriptional and translational levels. Our data also indicate that interstitial stem cells and their derivatives (e.g., neurons) have no major role in Hydra head regeneration. Remarkably, we found an enrichment of evolutionarily more recent genes in the early regeneration response, whereas conserved genes are more enriched in the late phase. In addition, genes specific to the early injury response were enriched in transposon insertions. Genetic dynamicity and taxon-specific factors might therefore play a hitherto underestimated role in Hydra regeneration.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Petersen, Hendrik O.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hoeger, Stefanie K.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Looso, MarioUNSPECIFIEDorcid.org/0000-0003-1495-9530UNSPECIFIED
Lengfeld, TobiasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kuhn, AnneUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Warnken, UweUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nishimiya-Fujisawa, ChiemiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schnoelzer, MartinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Krueger, MarcusUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Oezbek, SuatUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Simakov, OlegUNSPECIFIEDorcid.org/0000-0002-3585-4511UNSPECIFIED
Holstein, Thomas W.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-398209
DOI: 10.1093/molbev/msv079
Journal or Publication Title: Mol. Biol. Evol.
Volume: 32
Number: 8
Page Range: S. 1928 - 1948
Date: 2015
Publisher: OXFORD UNIV PRESS
Place of Publication: OXFORD
ISSN: 1537-1719
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
CONTROLS DNA-REPLICATION; DEVELOPMENTAL MECHANISMS; NEGATIVE REGULATORS; LIMB REGENERATION; GENETIC-ANALYSIS; HAIR FOLLICLE; CELL LINEAGES; AMINO-ACIDS; PROTEIN; FAMILYMultiple languages
Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & HeredityMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/39820

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