Universität zu Köln

Imhof, Thomas, Gruenewald, Nora, Schwarz, Guenter ORCID: 0000-0002-2118-9338, Noack, Michael J. and Koch, Manuel ORCID: 0000-0002-2962-7814 (2015). Modified amelogenin is a new and versatile nanomaterial for biomedical applications. Biotechnol. Bioeng., 112 (8). S. 1708 - 1714. HOBOKEN: WILEY. ISSN 1097-0290

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Abstract

Amelogenin self-assembly is crucial for tooth biomineralization and crystallite enamel orientation. Amelogenin forms stable nanoparticles under physiological conditions. Here, we tested whether the surface properties and binding characteristics of these particles could be modified to enhance amelogenin function as a biomaterial. We evaluated different amelogenin fusion proteins for their ability to form hybrid nanoparticles. As a proof-of-concept, the integrin-binding tripeptide Arg-Gly-Asp (RGD) sequence from fibronectin was integrated into mouse amelogenin (rM179) at three different positions. Dynamic light scattering (DLS) measurements revealed that these amelogenin fusion proteins still form nanospheres. Additional DLS and isothermal titration calorimetry measurements showed that the mixtures of RGD-modified amelogenin and wild-type amelogenin form stable particles. We determined that insertion of the RGD-loop at the amelogenin C-terminus converts the nanoparticle into a cell-binding substrate. Calvarial osteoblasts efficiently attached and spread on modified amelogenin, whereas almost no binding was observed on wild-type amelogenin. These results establish amelogenin as a new versatile biomaterial that can be easily modified to add additional functions. Biotechnol. Bioeng. 2015;112: 1708-1713. (c) 2015 Wiley Periodicals, Inc.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Imhof, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Gruenewald, Nora UNSPECIFIED UNSPECIFIED UNSPECIFIED Schwarz, Guenter UNSPECIFIED orcid.org/0000-0002-2118-9338 UNSPECIFIED Noack, Michael J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Koch, Manuel UNSPECIFIED orcid.org/0000-0002-2962-7814 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-398466
DOI:	10.1002/bit.25576
Journal or Publication Title:	Biotechnol. Bioeng.
Volume:	112
Number:	8
Page Range:	S. 1708 - 1714
Date:	2015
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1097-0290
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Chemistry > Institute of Biochemistry
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language Biotechnology & Applied Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/39846