van Wezel, Esther M., Stutterheim, Janine, Vree, Florentien, Zappeij-Kannegieter, Lily, Decarolis, Boris ORCID: 0000-0002-1607-0543, Hero, Barbara, Berthold, Frank, Schumacher-Kuckelkorn, Roswitha, Simon, Thorsten, Fiocco, Marta, Voermans, Carlijn, van Noesel, Max M., Caron, Huib N., van der Schoot, C. Ellen and Tytgat, Godelieve A. M. (2015). Minimal residual disease detection in autologous stem cell grafts from patients with high risk neuroblastoma. Pediatr. Blood Cancer, 62 (8). S. 1368 - 1374. HOBOKEN: WILEY-BLACKWELL. ISSN 1545-5017

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Abstract

BackgroundThe clinical significance of minimal residual disease (MRD) detected by real-time quantitative PCR (qPCR) in autologous stem cell grafts in high risk neuroblastoma is still controversial. In this retrospective multicenter study, autologous stem cell grafts of a large cohort were studied using a panel of RNA markers. ProcedureFrom 104 patients with high risk neuroblastoma, who received autologous stem cell transplantation as first line treatment, 66 peripheral blood stem cells (PBSC) and 38 CD34+ selected grafts were retrospectively collected at 2 Dutch and 12 German centers between 1997 and 2010. To investigate graft contamination qPCR was performed by using 5 neuroblastoma specific markers (PHOX2B, TH, DDC, CHRNA3, and DBH). ResultsIn PBSC 6/66 (9%) and in CD34+ selected grafts 3/38 (8%) samples were contaminated. Graft contamination was not associated with an unfavorable outcome (5-years OS, 66% vs. 50.5%; P=0.6 and 5-years EFS, 22% vs. 35%, P=0.7). In multivariate Cox analysis BM MRD at time of harvest was significantly associated with survival (P=0.008 OS and P=0.002 EFS), but graft contamination was still not associated with an unfavorable outcome (P=0.9 OS and P=1 EFS). ConclusionsGraft contamination is very infrequent in this retrospective cohort of patients with no or minimal BM disease prior to stem cell collection and does not influence outcome in univariate and multivariate analysis. The presence of MRD at time of harvest is a strong outcome predictor. However, these results will have to be verified in a large prospective study. Pediatr Blood Cancer 2015;62:1368-1373. (c) 2015 Wiley Periodicals, Inc.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
van Wezel, Esther M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Stutterheim, JanineUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Vree, FlorentienUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Zappeij-Kannegieter, LilyUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Decarolis, BorisUNSPECIFIEDorcid.org/0000-0002-1607-0543UNSPECIFIED
Hero, BarbaraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Berthold, FrankUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schumacher-Kuckelkorn, RoswithaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Simon, ThorstenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Fiocco, MartaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Voermans, CarlijnUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
van Noesel, Max M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Caron, Huib N.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
van der Schoot, C. EllenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Tytgat, Godelieve A. M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-398541
DOI: 10.1002/pbc.25507
Journal or Publication Title: Pediatr. Blood Cancer
Volume: 62
Number: 8
Page Range: S. 1368 - 1374
Date: 2015
Publisher: WILEY-BLACKWELL
Place of Publication: HOBOKEN
ISSN: 1545-5017
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
BONE-MARROW-TRANSPLANTATION; POLYMERASE-CHAIN-REACTION; STAGE IV NEUROBLASTOMA; PERIPHERAL-BLOOD; TUMOR-CELLS; CONTAMINATION; TIME; PCR; CONSENSUS; HARVESTSMultiple languages
Oncology; Hematology; PediatricsMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/39854

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