Universität zu Köln

Regenfuss, Birgit, Dreisow, Marie-Luise, Hos, Deniz, Masli, Sharmila, Bock, Felix and Cursiefen, Claus (2015). The Naive Murine Cornea as a Model System to Identify Novel Endogenous Regulators of Lymphangiogenesis: TRAIL and rtPA. Lymphat. Res. Biol., 13 (2). S. 76 - 85. NEW ROCHELLE: MARY ANN LIEBERT, INC. ISSN 1557-8585

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Abstract

Background: In the murine cornea, which is an established model for analyzing pathologic lymphatic vessel growth, phenotypic heterogeneity of the endogenous lymphatic vessels in the limbus of the cornea was previously described. In this study, the cornea of BALB/c, C57BL/6, and FVB mice with different limbal lymphangiogenic phenotypes was analyzed to identify novel candidates potentially influencing lymphatic vessel growth. Methods and Results: Pathway specific expression analysis of the cornea was performed to identify novel candidate genes. Corneal protein expression of the respective candidates was analyzed by fluorescent immunohistochemistry. The effect of the candidates on proliferation of human dermal lymphatic endothelial cells (HDLECs) was analyzed by BrdU proliferation ELISA. Thirteen genes were differentially regulated in corneas of mouse strains with more endogenous limbal lymphatic vessels (high-lymphangiogenic) (C57BL/6) compared to mouse strains with less endogenous limbal lymphatic vessels (low-lymphangiogenic) (BALB/c, FVB). Two candidates, Tumor necrosis factor (ligand) superfamily member 10 (Tnfsf10/Trail) and Plasminogen activator, tissue (Plat/tPA) were expressed in the cornea of BALB/c and C57BL/6 mice on the protein level. In vitro, Trail and recombinant tPA inhibited the proliferation of human dermal lymphatic endothelial cells. Conclusion: Molecular analysis of the naive cornea in mouse strains with different limbal lymphatic phenotypes is a valuable model to identify novel endogenous regulators of lymphangiogenesis.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Regenfuss, Birgit UNSPECIFIED UNSPECIFIED UNSPECIFIED Dreisow, Marie-Luise UNSPECIFIED UNSPECIFIED UNSPECIFIED Hos, Deniz UNSPECIFIED UNSPECIFIED UNSPECIFIED Masli, Sharmila UNSPECIFIED UNSPECIFIED UNSPECIFIED Bock, Felix UNSPECIFIED UNSPECIFIED UNSPECIFIED Cursiefen, Claus UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-401652
DOI:	10.1089/lrb.2015.0004
Journal or Publication Title:	Lymphat. Res. Biol.
Volume:	13
Number:	2
Page Range:	S. 76 - 85
Date:	2015
Publisher:	MARY ANN LIEBERT, INC
Place of Publication:	NEW ROCHELLE
ISSN:	1557-8585
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TISSUE-PLASMINOGEN-ACTIVATOR; APOPTOSIS-INDUCING LIGAND; ENDOTHELIAL-CELLS; GENETIC-HETEROGENEITY; MOUSE STRAINS; ANGIOGENESIS; GROWTH; VEGF; EXPRESSION; RECEPTOR-3 Multiple languages Medicine, Research & Experimental; Physiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/40165