Universität zu Köln

Sukumaran, Salil K., Blau-Wasser, Rosemarie, Rohlfs, Meino, Gallinger, Christoph, Schleicher, Michael and Noegel, Angelika A. (2015). The centrosomal component CEP161 of Dictyostelium discoideum interacts with the Hippo signaling pathway. Cell Cycle, 14 (7). S. 1024 - 1036. PHILADELPHIA: TAYLOR & FRANCIS INC. ISSN 1551-4005

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Abstract

CEP161 is a novel component of the Dictyostelium discoideum centrosome which was identified as binding partner of the pericentriolar component CP250. Here we show that the amino acids 1-763 of the 1381 amino acids CEP161 are sufficient for CP250 binding, centrosomal targeting and centrosome association. Analysis of AX2 cells over-expressing truncated and full length CEP161 proteins revealed defects in growth and development. By immunoprecipitation experiments we identified the Hippo related kinase SvkA (Hrk-svk) as binding partner for CEP161. Both proteins colocalize at the centrosome. In in vitro kinase assays the N-terminal domain of CEP161 (residues 1-763) inhibited the kinase activity of Hrk-svk. A comparison of D. discoideum Hippo kinase mutants with mutants overexpressing CEP161 polypeptides revealed similar defects. We propose that the centrosomal component CEP161 is a novel player in the Hippo signaling pathway and affects various cellular properties through this interaction.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Sukumaran, Salil K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Blau-Wasser, Rosemarie UNSPECIFIED UNSPECIFIED UNSPECIFIED Rohlfs, Meino UNSPECIFIED UNSPECIFIED UNSPECIFIED Gallinger, Christoph UNSPECIFIED UNSPECIFIED UNSPECIFIED Schleicher, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Noegel, Angelika A. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-403229
DOI:	10.1080/15384101.2015.1007015
Journal or Publication Title:	Cell Cycle
Volume:	14
Number:	7
Page Range:	S. 1024 - 1036
Date:	2015
Publisher:	TAYLOR & FRANCIS INC
Place of Publication:	PHILADELPHIA
ISSN:	1551-4005
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SITE-A GLYCOPROTEIN; CELL-CYCLE EXIT; PROLIFERATION ARREST; PROMOTES APOPTOSIS; DROSOPHILA; KINASE; GROWTH; HOMOLOG; PROTEINS; PHAGOCYTOSIS Multiple languages Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/40322