Rinschen, Markus M., Pahmeyer, Caroline, Pisitkun, Trairak, Schnell, Nicole, Wu, Xiongwu, Maass, Martina, Bartram, Malte P., Lamkemeyer, Tobias, Schermer, Bernhard ORCID: 0000-0002-5194-9000, Benzing, Thomas and Brinkkoetter, Paul T. (2015). Comparative phosphoproteomic analysis of mammalian glomeruli reveals conserved podocin C-terminal phosphorylation as a determinant of slit diaphragm complex architecture. Proteomics, 15 (7). S. 1326 - 1332. HOBOKEN: WILEY. ISSN 1615-9861

Full text not available from this repository.

Abstract

Glomerular biology is dependent on tightly controlled signal transduction networks that control phosphorylation of signaling proteins such as cytoskeletal regulators or slit diaphragm proteins of kidney podocytes. Cross-species comparison of phosphorylation events is a powerful mean to functionally prioritize and identify physiologically meaningful phosphorylation sites. Here, we present the result of phosphoproteomic analyses of cow and rat glomeruli to allow cross-species comparisons. We discovered several phosphorylation sites with potentially high biological relevance, e.g. tyrosine phosphorylation of the cytoskeletal regulator synaptopodin and the slit diaphragm protein neph-1 (Kirrel). Moreover, cross-species comparisons revealed conserved phosphorylation of the slit diaphragm protein nephrin on an acidic cluster at the intracellular terminus and conserved podocin phosphorylation on the very carboxyl terminus of the protein. We studied a highly conserved podocin phosphorylation site in greater detail and show that phosphorylation regulates affinity of the interaction with nephrin and CD2AP. Taken together, these results suggest that species comparisons of phosphoproteomic data may reveal regulatory principles in glomerular biology. All MS data have been deposited in the ProteomeXchange with identifier PXD001005 ().

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Rinschen, Markus M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Pahmeyer, CarolineUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Pisitkun, TrairakUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schnell, NicoleUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wu, XiongwuUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Maass, MartinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bartram, Malte P.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Lamkemeyer, TobiasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schermer, BernhardUNSPECIFIEDorcid.org/0000-0002-5194-9000UNSPECIFIED
Benzing, ThomasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Brinkkoetter, Paul T.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-403319
DOI: 10.1002/pmic.201400235
Journal or Publication Title: Proteomics
Volume: 15
Number: 7
Page Range: S. 1326 - 1332
Date: 2015
Publisher: WILEY
Place of Publication: HOBOKEN
ISSN: 1615-9861
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
ACTIN CYTOSKELETON; PROTEIN PODOCIN; KIDNEY; COMPONENT; NEPHRIN; PODOCYTESMultiple languages
Biochemical Research Methods; Biochemistry & Molecular BiologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/40331

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item