Weber, Stefanie, Hofmann, Andrea, Herms, Stefan ORCID: 0000-0002-2786-8200, Hoffmann, Per and Doerfler, Walter
(2015).
Destabilization of the human epigenome: consequences of foreign DNA insertions.
Epigenomics, 7 (5).
S. 745 - 756.
LONDON:
FUTURE MEDICINE LTD.
ISSN 1750-192X
Abstract
Aim: We previously reported changes of DNA methylation and transcription patterns in mammalian cells that carry integrated foreign DNA. Experiments were now designed to assess the epigenetic consequences of inserting a 5.6 kbp plasmid into the human genome. Methods: Differential transcription and CpG methylation patterns were compared between transgenomic and nontransgenomic cell clones by using gene chip microarray systems. Results: In 4.7% of the 28.869 gene segments analyzed, transcriptional activities were up-or downregulated in the transgenomic cell clones. Genome-wide profiling revealed differential methylation in 3791 of >480,000 CpG's examined in transgenomic versus nontransgenomic clones. Conclusion: The data document genome-wide effects of foreign DNA insertions on the epigenetic stability of human cells. Many fields in experimental biology and medicine employ transgenomic or otherwise genome-manipulated cells or organisms without considering the epigenetic consequences for the recipient genomes.
Item Type: | Journal Article | ||||||||||||||||||||||||
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URN: | urn:nbn:de:hbz:38-415819 | ||||||||||||||||||||||||
DOI: | 10.2217/EPI.15.40 | ||||||||||||||||||||||||
Journal or Publication Title: | Epigenomics | ||||||||||||||||||||||||
Volume: | 7 | ||||||||||||||||||||||||
Number: | 5 | ||||||||||||||||||||||||
Page Range: | S. 745 - 756 | ||||||||||||||||||||||||
Date: | 2015 | ||||||||||||||||||||||||
Publisher: | FUTURE MEDICINE LTD | ||||||||||||||||||||||||
Place of Publication: | LONDON | ||||||||||||||||||||||||
ISSN: | 1750-192X | ||||||||||||||||||||||||
Language: | English | ||||||||||||||||||||||||
Faculty: | Unspecified | ||||||||||||||||||||||||
Divisions: | Unspecified | ||||||||||||||||||||||||
Subjects: | no entry | ||||||||||||||||||||||||
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URI: | http://kups.ub.uni-koeln.de/id/eprint/41581 |
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