Universität zu Köln

Grau, Stefan J., Trillsch, Fabian ORCID: 0000-0002-1860-4350, Tonn, Joerg-Christian, Goldbrunner, Roland H., Noessner, Elfriede, Nelson, Peter J. and von Luettichau, Irene (2015). Podoplanin increases migration and angiogenesis in malignant glioma. Int. J. Clin. Exp. Pathol., 8 (7). S. 8663 - 8671. MADISON: E-CENTURY PUBLISHING CORP. ISSN 1936-2625

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Abstract

Expression of podoplanin in glial brain tumors is grade dependent. While serving as a marker for tumor progression and modulating invasion in various neoplasms, little is known about podoplanin function in gliomas. Therefore we stably transfected two human glioma cell lines (U373MG and U87MG) with expression plasmids encoding podoplanin. The efficacy of transfection was confirmed by FACS analysis, PCR and immunocytochemistry. Cells were then sorted for highly podoplanin expressing cells (U373P(high)/U87P(high)). Transfection did not influence the production of pro-angiogenic factors including VEGF, VEGF-C and D. Also, expression of VEGF receptors (VEGFR) remained unchanged except for U87P(high), where a VEGFR3 expression was induced. U373P(high) showed significantly reduced proliferation as compared to mock transfected group. By contrast, podoplanin significantly increased migration and invasion into collagen matrix. Furthermore, conditioned media from P-high glioma cells strongly induced tube formation on matrigel. In conclusion, podoplanin increased migration of tumor cells and enhanced tube formation activity in endothelial cells independent from VEGF. Thus, podoplanin expression may be an important step in tumor progression.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Grau, Stefan J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Trillsch, Fabian UNSPECIFIED orcid.org/0000-0002-1860-4350 UNSPECIFIED Tonn, Joerg-Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Goldbrunner, Roland H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Noessner, Elfriede UNSPECIFIED UNSPECIFIED UNSPECIFIED Nelson, Peter J. UNSPECIFIED UNSPECIFIED UNSPECIFIED von Luettichau, Irene UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-415923
Journal or Publication Title:	Int. J. Clin. Exp. Pathol.
Volume:	8
Number:	7
Page Range:	S. 8663 - 8671
Date:	2015
Publisher:	E-CENTURY PUBLISHING CORP
Place of Publication:	MADISON
ISSN:	1936-2625
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EXPRESSION; INVASION Multiple languages Oncology; Pathology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/41592