Universität zu Köln

Yin, Mengmeng, Yuan, Yin, Cui, Yurong, Hong, Xian, Luo, Hongyan, Hu, Xinwu, Tang, Ming, Hescheler, Jurgen and Xi, Jiaoya (2015). Puerarin Suppresses the Self-Renewal of Murine Embryonic Stem Cells by Inhibition of REST-MiR-21 Regulatory Pathway. Cell. Physiol. Biochem., 37 (2). S. 527 - 537. BASEL: KARGER. ISSN 1421-9778

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Abstract

Background/Aims: Puerarin shows a wide range of biological activities, including affecting the cardiac differentiation from murine embryonic stem (mES) cells. However, little is known about its effect and mechanism of action on the self-renewal of mES cells. This study aimed to determine the effect of puerarin on the self-renewal and pluripotency of mES cells and its underlying mechanisms. Methods: RT-PCR and real-time PCR were used to detect the transcripts of core transcription factors, specific markers for multiple lineages, REST and microRNA-21 (miR-21). Colony-forming assay was performed to estimate the self-renewal capacity of mES cells. Western blotting and wortmannin were employed to explore the role of PI3K/Akt signaling pathway in the inhibitory action of puerarin on REST transcript. Transfected mES cells with antagomir21 were used to confirm the role of miR-21 in the action of puerarin on cell self-renewal. Results: Puerarin significantly decreased the percentage of the self-renewal colonies, and suppressed the transcripts of Oct4, Nanog, Sox2, c-Myc and REST. Besides, PECAM, NCAM and miR-21 were up-regulated both under the self-renewal conditions and at day 4 of differentiation. The PI3K inhibitor wortmannin successfully reversed the mRNA expression changes of REST, Nanog and Sox2. Transfection of antagomir21 efficiently reversed the effects of puerarin on mES cells self-renewal. Conclusion: Inhibition of REST-miR-21 regulatory pathway may be the key mechanism of puerarin-induced suppression of mES cells self-renewal. Copyright (C) 2015 S. Karger AG, Basel

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Yin, Mengmeng UNSPECIFIED UNSPECIFIED UNSPECIFIED Yuan, Yin UNSPECIFIED UNSPECIFIED UNSPECIFIED Cui, Yurong UNSPECIFIED UNSPECIFIED UNSPECIFIED Hong, Xian UNSPECIFIED UNSPECIFIED UNSPECIFIED Luo, Hongyan UNSPECIFIED UNSPECIFIED UNSPECIFIED Hu, Xinwu UNSPECIFIED UNSPECIFIED UNSPECIFIED Tang, Ming UNSPECIFIED UNSPECIFIED UNSPECIFIED Hescheler, Jurgen UNSPECIFIED UNSPECIFIED UNSPECIFIED Xi, Jiaoya UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-415990
DOI:	10.1159/000430374
Journal or Publication Title:	Cell. Physiol. Biochem.
Volume:	37
Number:	2
Page Range:	S. 527 - 537
Date:	2015
Publisher:	KARGER
Place of Publication:	BASEL
ISSN:	1421-9778
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language UP-REGULATION; ES CELLS; DIFFERENTIATION; REST; PLURIPOTENCY; EXPRESSION; PI3K/AKT; CARDIOMYOCYTES; NETWORK; NEUROGENESIS Multiple languages Cell Biology; Physiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/41599