Luebke, Nadine, Di Cristanziano, Veronica, Sierra, Saleta, Knops, Elena, Schuelter, Eugen, Jensen, Bjorn, Oette, Mark, Lengauer, Thomas ORCID: 0000-0003-3801-2640 and Kaiser, Rolf (2015). Proviral DNA as a Target for HIV-1 Resistance Analysis. Intervirology, 58 (3). S. 184 - 190. BASEL: KARGER. ISSN 1423-0100

Full text not available from this repository.

Abstract

Background: Resistance analysis from viral RNA is restricted to detectable viral load. Therefore, analysis from proviral DNA could help in cases with low-level or suppressed viremia. Methods: Viral plasma RNA and the corresponding cellular proviral DNA of 78 EDTA samples from 48 therapy-naive (TN) and 30 therapy-experienced (TE) HIV-1-infected patients were isolated and analyzed for their resistance profiles in the protease and reverse transcriptase genes. Results: Overall, 175 drug-resistance mutations (DRMs) were detected in 25/30 TE (83.3%) and 5/48 TN (10.4%) samples. The TE patients displayed a mean number of 6.68 DRMs in RNA and 5.20 in DNA. In the TN patients, a mean of 0.8 DRMs was found in RNA and 1.0 in DNA; 75% of the DRMs were detected in RNA and DNA simultaneously. In the TE samples, 76% of the DRMs were detected simultaneously in RNA and DNA, 23% exclusively in RNA and 1% in DNA only. The TN samples revealed a significantly higher frequency of DRMs in DNA than in RNA. Conclusions: Proviral DNA resistance testing provides additional resistance information for TN patients. It is also a reliable alternative for TE patients with unsuccessful RNA testing and can provide valuable information when no records are available. (C) 2015 S. Karger AG, Basel

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Luebke, NadineUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Di Cristanziano, VeronicaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sierra, SaletaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Knops, ElenaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schuelter, EugenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Jensen, BjornUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Oette, MarkUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Lengauer, ThomasUNSPECIFIEDorcid.org/0000-0003-3801-2640UNSPECIFIED
Kaiser, RolfUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-416166
DOI: 10.1159/000431093
Journal or Publication Title: Intervirology
Volume: 58
Number: 3
Page Range: S. 184 - 190
Date: 2015
Publisher: KARGER
Place of Publication: BASEL
ISSN: 1423-0100
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
LOW-LEVEL VIREMIA; ACTIVE ANTIRETROVIRAL THERAPY; BLOOD MONONUCLEAR-CELLS; GENOTYPIC RESISTANCE; EXPERIENCED PATIENTS; MUTATIONS; PLASMA; NAIVE; EVOLUTION; RESERVOIRMultiple languages
VirologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/41616

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item