Isachenko, V., Isachenko, E., Sanchez, R., Dattena, M., Mallmann, P. and Rahimi, G. (2015). Cryopreservation of Whole Ovine Ovaries with Pedicles as a Model for Human: Parameters of Perfusion with Simultaneous Saturations by Cryoprotectants. Clin. Lab., 61 (3-4). S. 415 - 421. HEIDELBERG: CLIN LAB PUBL. ISSN 1433-6510
Full text not available from this repository.Abstract
Background: The aim of this research was to study the effectiveness of perfusion of intact ovine ovaries with different rates of perfusion and time-period elapsed between extraction of these ovaries and the beginning of perfusion. Methods: Ovaries were perfused through the arteria ovarica (ovarian arteries) with culture medium supplemented with 5% bovine calf serum, 6% dimethyl sulfoxide, 6% ethylene glycol, 0.15M sucrose, Indian ink, and 100 IU/mL heparin at room temperature (22 degrees C). In the first cycle of experiments, ovaries (n = 96) were perfused for 60 minutes just after extraction of ovaries at the following rates of perfusion (mL/hour): 150, 100, 75, 50, 25, 12.5, and 6.3. In the second cycle of experiments, ovaries (n = 26) were perfused at a rate of 25 mL/hour for 60 minutes after extraction of ovaries and their storage at room temperature for 2, 3, 4, and 5 hours, for groups 1, 2, 3, and, 4, respectively. Successful perfusion of blood vessels was detected visible by a blue coloration of the ovarian tissues. Results: The first cycle of experiments showed that the optimal perfusion rates were 50 mL/hour and 25 mL/hour. In the second cycle of experiments, good perfusion of ovaries was established at a perfusion rate of 25 mL/hour for the ovaries of groups when 2 and 3 hours had elapsed after extraction. Conclusions: Effective perfusion of ovine intact ovaries with vascular pedicle was established using freezing medium at room temperature at the rate of perfusion of 25 mL/hour or 50 mL/hour. The ovaries must be perfused not later than 3 hours after the death of animals.
Item Type: | Journal Article | ||||||||||||||||||||||||||||
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URN: | urn:nbn:de:hbz:38-416768 | ||||||||||||||||||||||||||||
DOI: | 10.7754/Clin.Lab.2014.140919 | ||||||||||||||||||||||||||||
Journal or Publication Title: | Clin. Lab. | ||||||||||||||||||||||||||||
Volume: | 61 | ||||||||||||||||||||||||||||
Number: | 3-4 | ||||||||||||||||||||||||||||
Page Range: | S. 415 - 421 | ||||||||||||||||||||||||||||
Date: | 2015 | ||||||||||||||||||||||||||||
Publisher: | CLIN LAB PUBL | ||||||||||||||||||||||||||||
Place of Publication: | HEIDELBERG | ||||||||||||||||||||||||||||
ISSN: | 1433-6510 | ||||||||||||||||||||||||||||
Language: | English | ||||||||||||||||||||||||||||
Faculty: | Unspecified | ||||||||||||||||||||||||||||
Divisions: | Unspecified | ||||||||||||||||||||||||||||
Subjects: | no entry | ||||||||||||||||||||||||||||
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URI: | http://kups.ub.uni-koeln.de/id/eprint/41676 |
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