Kock, Malte, Nunes, Maria M., Hemann, Matthias, Kube, Sebastian, Dohmen, R. Juergen, Herzog, Franz, Ramos, Paula C. and Wendler, Petra ORCID: 0000-0002-8285-2199 (2015). Proteasome assembly from 15S precursors involves major conformational changes and recycling of the Pba1-Pba2 chaperone. Nat. Commun., 6. LONDON: NATURE PUBLISHING GROUP. ISSN 2041-1723

Full text not available from this repository.

Abstract

The chaperones Ump1 and Pba1-Pba2 promote efficient biogenesis of 20S proteasome core particles from its subunits via 15S intermediates containing alpha and beta subunits, except beta7. Here we elucidate the structural role of these chaperones in late steps of core particle biogenesis using biochemical, electron microscopy, cross-linking and mass spectrometry analyses. In 15S precursor complexes, Ump1 is largely unstructured, lining the inner cavity of the complex along the interface between alpha and beta subunits. The alpha and beta subunits form loosely packed rings with a wider alpha ring opening than in the 20S core particle, allowing for the Pba1-Pba2 heterodimer to be partially embedded in the central alpha ring cavity. During biogenesis, the heterodimer is expelled from the alpha ring by a restructuring event that organizes the beta ring and leads to tightening of the alpha ring opening. In this way, the Pba1-Pba2 chaperone is recycled for a new round of proteasome assembly.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Kock, MalteUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nunes, Maria M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hemann, MatthiasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kube, SebastianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Dohmen, R. JuergenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Herzog, FranzUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ramos, Paula C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wendler, PetraUNSPECIFIEDorcid.org/0000-0002-8285-2199UNSPECIFIED
URN: urn:nbn:de:hbz:38-417576
DOI: 10.1038/ncomms7123
Journal or Publication Title: Nat. Commun.
Volume: 6
Date: 2015
Publisher: NATURE PUBLISHING GROUP
Place of Publication: LONDON
ISSN: 2041-1723
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
20S PROTEASOME; STRUCTURAL BASIS; NEW-GENERATION; SUBUNIT; YEAST; COMPLEX; MATURATION; SUBSTRATE; SYSTEM; GENEMultiple languages
Multidisciplinary SciencesMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/41757

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item