Universität zu Köln

Overkamp, Kristina E., Langklotz, Sina, Aras, Marco, Helling, Stefan, Marcus, Katrin, Bandow, Julia E., Hoef-Emden, Kerstin and Frankenberg-Dinkel, Nicole ORCID: 0000-0002-7757-6839 (2014). Chromophore composition of the phycobiliprotein Cr-PC577 from the cryptophyte Hemiselmis pacifica. Photosynth. Res., 122 (3). S. 293 - 305. DORDRECHT: SPRINGER. ISSN 1573-5079

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Abstract

The cryptophyte phycocyanin Cr-PC577 from Hemiselmis pacifica is a close relative of Cr-PC612 found in Hemiselmis virescens and Hemiselmis tepida. The two biliproteins differ in that Cr-PC577 lacks the major peak at around 612 nm in the absorption spectrum. Cr-PC577 was thus purified and characterized with respect to its bilin chromophore composition. Like other cryptophyte phycobiliproteins, Cr-PC577 is an (alpha beta)(alpha'beta) heterodimer with phycocyanobilin (PCB) bound to the alpha-subunits. While one chromophore of the beta-subunit is also PCB, mass spectrometry identified an additional chromophore with a mass of 585 Da at position beta-Cys-158. This mass can be attributed to either a dihydrobiliverdin (DHBV), mesobiliverdin (MBV), or bilin584 chromophore. The doubly linked bilin at position beta-Cys-50 and beta-Cys-61 could not be identified unequivocally but shares spectral features with DHBV. We found that Cr-PC577 possesses a novel chromophore composition with at least two different chromophores bound to the beta-subunit. Overall, our data contribute to a better understanding of cryptophyte phycobiliproteins and furthermore raise the question on the biosynthetic pathway of cryptophyte chromophores.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Overkamp, Kristina E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Langklotz, Sina UNSPECIFIED UNSPECIFIED UNSPECIFIED Aras, Marco UNSPECIFIED UNSPECIFIED UNSPECIFIED Helling, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Marcus, Katrin UNSPECIFIED UNSPECIFIED UNSPECIFIED Bandow, Julia E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoef-Emden, Kerstin UNSPECIFIED UNSPECIFIED UNSPECIFIED Frankenberg-Dinkel, Nicole UNSPECIFIED orcid.org/0000-0002-7757-6839 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-422178
DOI:	10.1007/s11120-014-0029-1
Journal or Publication Title:	Photosynth. Res.
Volume:	122
Number:	3
Page Range:	S. 293 - 305
Date:	2014
Publisher:	SPRINGER
Place of Publication:	DORDRECHT
ISSN:	1573-5079
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language BILIVERDIN-IX-ALPHA; CRYPTOMONAD BILIPROTEINS; MARINE-ALGAE; PHYCOCYANIN; BIOSYNTHESIS; EVOLUTION; PROTEINS; DIHYDROBILIVERDIN; SEPARATION; SEQUENCE Multiple languages Plant Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/42217