Nolte, Hendrik, Hoelper, Soraya, Selbach, Matthias, Braun, Thomas ORCID: 0000-0002-6165-4804 and Krueger, Marcus (2014). Assessment of Serum Protein Dynamics by Native SILAC Flooding (SILflood). Anal. Chem., 86 (22). S. 11033 - 11038. WASHINGTON: AMER CHEMICAL SOC. ISSN 1520-6882

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Abstract

Turnover of blood serum proteins is a vital function in mammals, but technical challenges have thus far prevented comprehensive measurements of serum protein half-lives. Here, we injected native serum from heavy stable isotope labeled (Sit) mice into nonlabeled recipients to quantify turnover of more than 200 proteins using mass spectrometry with high reproducibility and accuracy. We found a median of 19.4 h and a total range of 6-70 h for calculated half-lives. Moreover, we observed similar half-lives for proteins with equal function. To demonstrate the value and effectiveness of SILflood, we investigated the impaired serum clearance in beta 2-microglobulin (B2M-/-) deficient mice. Notably, we found that serum albumin and IgG half-lives were clearly reduced in B2M deficient animals compared to control animals. Taken together, our results demonstrate that SILflood is a versatile tool to investigate serum half-lives under regular and pathological conditions in living animals.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Nolte, HendrikUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hoelper, SorayaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Selbach, MatthiasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Braun, ThomasUNSPECIFIEDorcid.org/0000-0002-6165-4804UNSPECIFIED
Krueger, MarcusUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-423142
DOI: 10.1021/ac502883p
Journal or Publication Title: Anal. Chem.
Volume: 86
Number: 22
Page Range: S. 11033 - 11038
Date: 2014
Publisher: AMER CHEMICAL SOC
Place of Publication: WASHINGTON
ISSN: 1520-6882
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
TURNOVER; ALBUMIN; IGGMultiple languages
Chemistry, AnalyticalMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/42314

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