Universität zu Köln

Mommaerts, Hendrik, Esguerra, Camila V., Hartmann, Ursula, Luyten, Frank P. and Tylzanowski, Przemko ORCID: 0000-0003-0769-559X (2014). Smoc2 Modulates Embryonic Myelopoiesis During Zebrafish Development. Dev. Dyn., 243 (11). S. 1375 - 1391. HOBOKEN: WILEY-BLACKWELL. ISSN 1097-0177

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Abstract

Background: SMOC2 is a member of the BM-40 (SPARC) family of matricellular proteins, reported to influence signaling in the extracellular compartment. In mice, Smoc2 is expressed in many different tissues and was shown to enhance the response to angiogenic growth factors, mediate cell adhesion, keratinocyte migration, and metastasis. Additionally, SMOC2 is associated with vitiligo and craniofacial and dental defects. The function of Smoc2 during early zebrafish development has not been determined to date. Results: In pregastrula zebrafish embryos, smoc2 is expressed ubiquitously. As development progresses, the expression pattern becomes more anteriorly restricted. At the onset of blood cell circulation, smoc2 morphants presented a mild ventralization of posterior structures. Molecular analysis of the smoc2 morphants indicated myelopoietic defects in the rostral blood islands during segmentation stages. Hemangioblast development and further specification of the myeloid progenitor cells were shown to be impaired. Additional experiments indicated that Bmp target genes were down-regulated in smoc2 morphants. Conclusions: Our findings reveal that Smoc2 is an essential player in the development of myeloid cells of the anterior lateral plate mesoderm during embryonic zebrafish development. Furthermore, our data show that Smoc2 affects the transcription of Bmp target genes without affecting initial dorsoventral patterning or mesoderm development. Developmental Dynamics 243:1375-1390, 2014. (c) 2014 Wiley Periodicals, Inc.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Mommaerts, Hendrik UNSPECIFIED UNSPECIFIED UNSPECIFIED Esguerra, Camila V. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hartmann, Ursula UNSPECIFIED UNSPECIFIED UNSPECIFIED Luyten, Frank P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Tylzanowski, Przemko UNSPECIFIED orcid.org/0000-0003-0769-559X UNSPECIFIED
URN:	urn:nbn:de:hbz:38-424626
DOI:	10.1002/dvdy.24164
Journal or Publication Title:	Dev. Dyn.
Volume:	243
Number:	11
Page Range:	S. 1375 - 1391
Date:	2014
Publisher:	WILEY-BLACKWELL
Place of Publication:	HOBOKEN
ISSN:	1097-0177
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CALCIUM-BINDING PROTEIN; PRIMITIVE MYELOPOIESIS; EXPRESSION; GENE; SCL; HEMATOPOIESIS; GROWTH; BLOOD; ALK8; FATE Multiple languages Anatomy & Morphology; Developmental Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/42462