Kuhn, Sebastian, Kroth, Jennifer, Ritz, Ulrike, Hofmann, Alexander, Brendel, Christian ORCID: 0000-0003-4081-4351, Mueller, Lars Peter, Foerch, Renate and Rommens, Pol Maria (2014). Reduced fibroblast adhesion and proliferation on plasma-modified titanium surfaces. J. Mater. Sci.-Mater. Med., 25 (11). S. 2549 - 2561. DORDRECHT: SPRINGER. ISSN 1573-4838

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Abstract

Soft tissue complications are clinically relevant problems after osteosynthesis of fractures. The goal is to develop a method for reduction of fibroblast adhesion and proliferation on titanium implant surfaces by plasma polymerisation of the organo-silicon monomer hexamethyldisiloxane (HMDSO). HMDSO was deposited under continuous wave conditions in excess oxygen (ppHMDSO surface) and selected samples were further modified with an additional oxygen plasma (ppHMDSO + O-2 surface). Surface characterization was performed by scanning electron microscopy, profilometry, water contact angle measurements, infrared reflection absorption spectroscopy and X-ray photoelectron spectroscopy. In our experimental setup the mechanical properties, roughness and topography of the titanium were preserved, while surface chemistry was drastically changed. Fibroblast proliferation was assessed by alamarBlue assay, cell morphology by confocal microscopy visualization of eGFP-transducted fibroblasts, and cell viability by Annexine V/propidium iodide assay. Both modified surfaces, non-activated hydrophobic ppHMDSO and activated hydrophilic ppHMDSO + O-2 were able to dramatically reduce fibroblast colonization and proliferation compared to standard titanium. However, this effect was more strongly pronounced on the hydrophobic ppHMDSO surface, which caused reduced cell adhesion and prevented proliferation of fibroblasts. The results demonstrate that plasma modifications of titanium using HMDSO are valuable candidates for future developments in anti-adhesive and anti-proliferative coatings for titanium fracture implants.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Kuhn, SebastianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kroth, JenniferUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ritz, UlrikeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hofmann, AlexanderUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Brendel, ChristianUNSPECIFIEDorcid.org/0000-0003-4081-4351UNSPECIFIED
Mueller, Lars PeterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Foerch, RenateUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rommens, Pol MariaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-424700
DOI: 10.1007/s10856-014-5278-1
Journal or Publication Title: J. Mater. Sci.-Mater. Med.
Volume: 25
Number: 11
Page Range: S. 2549 - 2561
Date: 2014
Publisher: SPRINGER
Place of Publication: DORDRECHT
ISSN: 1573-4838
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
IN-VIVO EVALUATION; TISSUE ADHESION; CELL-SHAPE; HEXAMETHYLDISILOXANE; FILMS; WETTABILITY; IMPROVEMENT; REMOVAL; BIOCOMPATIBILITY; POLYMERIZATIONMultiple languages
Engineering, Biomedical; Materials Science, BiomaterialsMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/42470

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