Frobel, Joana, Hemeda, Hatim, Lenz, Michael ORCID: 0000-0002-6319-6480, Abagnale, Giulio, Joussen, Sylvia, Denecke, Bernd, Saric, Tomo ORCID: 0000-0001-8344-1095, Zenke, Martin and Wagner, Wolfgang ORCID: 0000-0002-1971-3217 (2014). Epigenetic Rejuvenation of Mesenchymal Stromal Cells Derived from Induced Pluripotent Stem Cells. Stem Cell Rep., 3 (3). S. 414 - 423. CAMBRIDGE: CELL PRESS. ISSN 2213-6711

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Abstract

Standardization of mesenchymal stromal cells (MSCs) remains a major obstacle in regenerative medicine. Starting material and culture expansion affect cell preparations and render comparison between studies difficult. In contrast, induced pluripotent stem cells (iPSCs) assimilate toward a ground state and may therefore give rise to more standardized cell preparations. We reprogrammed MSCs into iPSCs, which were subsequently redifferentiated toward MSCs. These iPS-MSCs revealed similar morphology, immunophenotype, in vitro differentiation potential, and gene expression profiles as primary MSCs. However, iPS-MSCs were impaired in suppressing T cell proliferation. DNA methylation (DNAm) profiles of iPSCs maintained donor-specific characteristics, whereas tissue-specific, senescence-associated, and age-related DNAm patterns were erased during reprogramming. iPS-MSCs reacquired senescence-associated DNAm during culture expansion, but they remained rejuvenated with regard to age-related DNAm. Overall, iPS-MSCs are similar to MSCs, but they reveal incomplete reacquisition of immunomodulatory function and MSC-specific DNAm patterns-particularly of DNAm patterns associated with tissue type and aging.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Frobel, JoanaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hemeda, HatimUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Lenz, MichaelUNSPECIFIEDorcid.org/0000-0002-6319-6480UNSPECIFIED
Abagnale, GiulioUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Joussen, SylviaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Denecke, BerndUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Saric, TomoUNSPECIFIEDorcid.org/0000-0001-8344-1095UNSPECIFIED
Zenke, MartinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wagner, WolfgangUNSPECIFIEDorcid.org/0000-0002-1971-3217UNSPECIFIED
URN: urn:nbn:de:hbz:38-428842
DOI: 10.1016/j.stemcr.2014.07.003
Journal or Publication Title: Stem Cell Rep.
Volume: 3
Number: 3
Page Range: S. 414 - 423
Date: 2014
Publisher: CELL PRESS
Place of Publication: CAMBRIDGE
ISSN: 2213-6711
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
DNA METHYLATION CHANGES; CPG SITES; SENESCENCE; DONORMultiple languages
Cell & Tissue Engineering; Cell BiologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/42884

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