Universität zu Köln

Appel, S., Turnwald, E-M., Alejandre-Alcazar, M. A., Ankerne, J., Rother, E., Janoschek, R., Wohlfarth, M., Vohlen, C., Schnare, M., Meissner, U. and Doetsch, J. (2014). Leptin does not Induce an Inflammatory Response in the Murine Placenta. Horm. Metab. Res., 46 (6). S. 384 - 390. STUTTGART: GEORG THIEME VERLAG KG. ISSN 1439-4286

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Abstract

Leptin is described as a pro-inflammatory signal in fat tissue, which is released from adipocytes and in turn activates immune cells. Also, leptin levels are known to be increased in pregnancies complicated with enhanced inflammatory processes in the placenta. Hence, we assumed that increased leptin amounts might contribute to inducing an inflammatory response in the placenta. To test this hypothesis, pregnant mice were continuously infused with recombinant murine leptin s.c. from day g13 to g16, resulting in a 3-fold increase of maternal circulating serum leptin levels. Dissected placentas were examined for the expression of pro-inflammatory cytokines IL-6 and TNF-alpha and the anti-inflammatory cytokine IL-10 using qPCR analysis. No changes were found except for TNF-alpha, which was slightly elevated upon leptin stimulation. However, TNF-alpha protein levels were not significantly higher in placentas from leptin treated mice. Also, leukocyte infiltration in the labyrinth section of placentas was not increased. In summary, our data demonstrate for the first time that elevated leptin levels alone do not induce an inflammatory response in the placenta.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Appel, S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Turnwald, E-M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Alejandre-Alcazar, M. A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ankerne, J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Rother, E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Janoschek, R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Wohlfarth, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Vohlen, C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schnare, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Meissner, U. UNSPECIFIED UNSPECIFIED UNSPECIFIED Doetsch, J. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-437041
DOI:	10.1055/s-0034-1368710
Journal or Publication Title:	Horm. Metab. Res.
Volume:	46
Number:	6
Page Range:	S. 384 - 390
Date:	2014
Publisher:	GEORG THIEME VERLAG KG
Place of Publication:	STUTTGART
ISSN:	1439-4286
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TROPHOBLAST CELLS; PREGNANCY; EXPRESSION; SECRETION; CYTOKINES; OBESITY; WEIGHT; MICE Multiple languages Endocrinology & Metabolism Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/43704