van den Berge, M., Carracedo, A., Gomes, I., Graham, E. A. M., Haas, C., Hjort, B., Hoff-Olsen, P., Maronas, O., Mevag, B., Morling, N., Niederstaetter, H., Parson, W., Schneider, P. M., Court, D. Syndercombe, Vidaki, A. and Sijen, T. (2014). A collaborative European exercise on mRNA-based body fluid/skin typing and interpretation of DNA and RNA results. Forensic Sci. Int.-Genet., 10. S. 40 - 49. CLARE: ELSEVIER IRELAND LTD. ISSN 1878-0326

Full text not available from this repository.

Abstract

The European Forensic Genetics Network of Excellence (EUROFORGEN-NoE) undertook a collaborative project on mRNA-based body fluid/skin typing and the interpretation of the resulting RNA and DNA data. Although both body fluids and skin are composed of a variety of cell types with different functions and gene expression profiles, we refer to the procedure as 'cell type inference'. Nine laboratories participated in the project and used a 20-marker multiplex to analyse samples that were centrally prepared and thoroughly tested prior to shipment. Specimens of increasing complexity were assessed that ranged from reference PCR products, cDNAs of indicated or unnamed cell type source(s), to challenging mock casework stains. From this specimen set, information on the overall sensitivity and specificity of the various markers was obtained. In addition, the reliability of a scoring system for inference of cell types was assessed. This scoring system builds on replicate RNA analyses and the ratio observed/possible peaks for each cell type [1]. The results of the exercise support the usefulness of this scoring system. When interpreting the data obtained from the analysis of the mock casework stains, the participating laboratories were asked to integrate the DNA and RNA results and associate donor and cell type where possible. A large variation for the integrated interpretations of the DNA and RNA data was obtained including correct interpretations. We infer that with expertise in analysing RNA profiles, clear guidelines for data interpretation and awareness regarding potential pitfalls in associating donors and cell types, mRNA-based cell type inference can be implemented for forensic casework. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
van den Berge, M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Carracedo, A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gomes, I.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Graham, E. A. M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Haas, C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hjort, B.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hoff-Olsen, P.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Maronas, O.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mevag, B.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Morling, N.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Niederstaetter, H.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Parson, W.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schneider, P. M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Court, D. SyndercombeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Vidaki, A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sijen, T.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-439996
DOI: 10.1016/j.fsigen.2014.01.006
Journal or Publication Title: Forensic Sci. Int.-Genet.
Volume: 10
Page Range: S. 40 - 49
Date: 2014
Publisher: ELSEVIER IRELAND LTD
Place of Publication: CLARE
ISSN: 1878-0326
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
RNA/DNA CO-ANALYSIS; QUANTITATIVE RT-PCR; EDNAP EXERCISE; VAGINAL SECRETIONS; MENSTRUAL BLOOD; SALIVA STAINS; SEMEN STAINS; IDENTIFICATION; MULTIPLEX; BIOMARKERSMultiple languages
Genetics & Heredity; Medicine, LegalMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/43999

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item