Universität zu Köln

Aubry, Sylvain ORCID: 0000-0002-7598-3609, Smith-Unna, Richard D., Boursnell, Chris M., Kopriva, Stanislav ORCID: 0000-0002-7416-6551 and Hibberd, Julian M. (2014). Transcript residency on ribosomes reveals a key role for the Arabidopsis thaliana bundle sheath in sulfur and glucosinolate metabolism. Plant J., 78 (4). S. 659 - 674. HOBOKEN: WILEY. ISSN 1365-313X

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Abstract

Leaves of angiosperms are made up of multiple distinct cell types. While the function of mesophyll cells, guard cells, phloem companion cells and sieve elements are clearly described, this is not the case for the bundle sheath (BS). To provide insight into the role of the BS in the C-3 species Arabidopsis thaliana, we labelled ribosomes in this cell type with a FLAG tag. We then used immunocapture to isolate these ribosomes, followed by sequencing of resident mRNAs. This showed that 5% of genes showed specific splice forms in the BS, and that 15% of genes were preferentially expressed in these cells. The BS translatome strongly implies that the BS plays specific roles in sulfur transport and metabolism, glucosinolate biosynthesis and trehalose metabolism. Much of the C-4 cycle is differentially expressed between the C-3 BS and the rest of the leaf. Furthermore, the global patterns of transcript residency on BS ribosomes overlap to a greater extent with cells of the root pericycle than any other cell type. This analysis provides the first insight into the molecular function of this cell type in C-3 species, and also identifies characteristics of BS cells that are probably ancestral to both C-3 and C-4 plants.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Aubry, Sylvain UNSPECIFIED orcid.org/0000-0002-7598-3609 UNSPECIFIED Smith-Unna, Richard D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Boursnell, Chris M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kopriva, Stanislav UNSPECIFIED orcid.org/0000-0002-7416-6551 UNSPECIFIED Hibberd, Julian M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-440273
DOI:	10.1111/tpj.12502
Journal or Publication Title:	Plant J.
Volume:	78
Number:	4
Page Range:	S. 659 - 674
Date:	2014
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1365-313X
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language C-4 PHOTOSYNTHESIS; SULFATE ASSIMILATION; ASCORBATE-PEROXIDASE-2 EXPRESSION; FUNCTIONAL-DIFFERENTIATION; GLYCINE DECARBOXYLASE; MESOPHYLL PROTOPLASTS; GLUTATHIONE SYNTHESIS; FLAVERIA-TRINERVIA; LEAF SENESCENCE; CELLS Multiple languages Plant Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/44027