Universität zu Köln

Kirn, Verena, Zaharieva, Ivanka, Heublein, Sabine, Thangarajah, Fabinshy, Friese, Klaus, Mayr, Doris and Jeschke, Udo (2014). ESR1 Promoter Methylation in Squamous Cell Cervical Cancer. Anticancer Res., 34 (2). S. 723 - 728. ATHENS: INT INST ANTICANCER RESEARCH. ISSN 1791-7530

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Abstract

Background/Aim: Estrogen receptor-alpha is usually expressed in normal cervical tissue, but its presence is decreased or absent in invasive cervical cancer indicating that its expression is lost during development of invasive cervical cancer. The aim of the present study was to investigate ESR1 promoter methylation in cervical cancer and correlate methylation status with clinico-pathological parameters. Materials and Methods: Fifty patients treated for cervical cancer were included in the study. Isolation and bisulfite treatment of genomic DNA from cervical cancer tissue was performed by commercially-available kits. Methylated ESR1 promoter sequences were detected by quantitative real-time methylation-specific PCR. Results: Methylation status did not present differences regarding age at-diagnosis, FIGO stage, grade, BMI and overall survival for all patients, but within the subgroup of non-keratinizing squamous cell cancer methylation status correlated with grading (p=0.047). Conclusion: Methylation of the ESR1 promoter does not seem to be of any prognostic relevance, but is associated with higher tumor grading of cervical cancer patients.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kirn, Verena UNSPECIFIED UNSPECIFIED UNSPECIFIED Zaharieva, Ivanka UNSPECIFIED UNSPECIFIED UNSPECIFIED Heublein, Sabine UNSPECIFIED UNSPECIFIED UNSPECIFIED Thangarajah, Fabinshy UNSPECIFIED UNSPECIFIED UNSPECIFIED Friese, Klaus UNSPECIFIED UNSPECIFIED UNSPECIFIED Mayr, Doris UNSPECIFIED UNSPECIFIED UNSPECIFIED Jeschke, Udo UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-446969
Journal or Publication Title:	Anticancer Res.
Volume:	34
Number:	2
Page Range:	S. 723 - 728
Date:	2014
Publisher:	INT INST ANTICANCER RESEARCH
Place of Publication:	ATHENS
ISSN:	1791-7530
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CPG-ISLAND METHYLATION; DNA METHYLATION; ER-ALPHA; ESTROGEN; HYPERMETHYLATION; NEOPLASIA; GENE; EXPRESSION; CARCINOMA Multiple languages Oncology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/44696