Khatri, Priti, Obernier, Kirsten ORCID: 0000-0002-4025-1299, Simeonova, Ina K., Hellwig, Andrea, Hoelzl-Wenig, Gabriele, Mandl, Claudia, Scholl, Catharina, Woelfl, Stefan, Winkler, Johannes, Gaspar, John A., Sachinidis, Agapios and Ciccolini, Francesca
ORCID: 0000-0002-7276-7361
(2014).
Proliferation and cilia dynamics in neural stem cells prospectively isolated from the SEZ.
Sci Rep, 4.
BERLIN:
NATURE RESEARCH.
ISSN 2045-2322
Abstract
Neural stem cells (NSCs) generate new neurons in vivo and in vitro throughout adulthood and therefore are physiologically and clinically relevant. Unveiling the mechanisms regulating the lineage progression from NSCs to newborn neurons is critical for the transition from basic research to clinical application. However, the direct analysis of NSCs and their progeny is still elusive due to the problematic identification of the cells. We here describe the isolation of highly purified genetically unaltered NSCs and transit-amplifying precursors (TAPs) from the adult subependymal zone (SEZ). Using this approach we show that a primary cilium and high levels of epidermal growth factor receptor (EGFR) at the cell membrane characterize quiescent and cycling NSCs, respectively. However, we also observed non-ciliated quiescent NSCs and NSCs progressing into the cell cycle without up-regulating EGFR expression. Thus, the existence of NSCs displaying distinct molecular and structural conformations provides more flexibility to the regulation of quiescence and cell cycle progression.
Item Type: | Journal Article | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Creators: |
|
||||||||||||||||||||||||||||||||||||||||||||||||||||
URN: | urn:nbn:de:hbz:38-448297 | ||||||||||||||||||||||||||||||||||||||||||||||||||||
DOI: | 10.1038/srep03803 | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Journal or Publication Title: | Sci Rep | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Volume: | 4 | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Date: | 2014 | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Publisher: | NATURE RESEARCH | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Place of Publication: | BERLIN | ||||||||||||||||||||||||||||||||||||||||||||||||||||
ISSN: | 2045-2322 | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Language: | English | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Faculty: | Unspecified | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Divisions: | Unspecified | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Subjects: | no entry | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Uncontrolled Keywords: |
|
||||||||||||||||||||||||||||||||||||||||||||||||||||
URI: | http://kups.ub.uni-koeln.de/id/eprint/44829 |
Downloads
Downloads per month over past year
Altmetric
Export
Actions (login required)
![]() |
View Item |