Universität zu Köln

Khatri, Priti, Obernier, Kirsten ORCID: 0000-0002-4025-1299, Simeonova, Ina K., Hellwig, Andrea, Hoelzl-Wenig, Gabriele, Mandl, Claudia, Scholl, Catharina, Woelfl, Stefan, Winkler, Johannes, Gaspar, John A., Sachinidis, Agapios and Ciccolini, Francesca ORCID: 0000-0002-7276-7361 (2014). Proliferation and cilia dynamics in neural stem cells prospectively isolated from the SEZ. Sci Rep, 4. BERLIN: NATURE RESEARCH. ISSN 2045-2322

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Abstract

Neural stem cells (NSCs) generate new neurons in vivo and in vitro throughout adulthood and therefore are physiologically and clinically relevant. Unveiling the mechanisms regulating the lineage progression from NSCs to newborn neurons is critical for the transition from basic research to clinical application. However, the direct analysis of NSCs and their progeny is still elusive due to the problematic identification of the cells. We here describe the isolation of highly purified genetically unaltered NSCs and transit-amplifying precursors (TAPs) from the adult subependymal zone (SEZ). Using this approach we show that a primary cilium and high levels of epidermal growth factor receptor (EGFR) at the cell membrane characterize quiescent and cycling NSCs, respectively. However, we also observed non-ciliated quiescent NSCs and NSCs progressing into the cell cycle without up-regulating EGFR expression. Thus, the existence of NSCs displaying distinct molecular and structural conformations provides more flexibility to the regulation of quiescence and cell cycle progression.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Khatri, Priti UNSPECIFIED UNSPECIFIED UNSPECIFIED Obernier, Kirsten UNSPECIFIED orcid.org/0000-0002-4025-1299 UNSPECIFIED Simeonova, Ina K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hellwig, Andrea UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoelzl-Wenig, Gabriele UNSPECIFIED UNSPECIFIED UNSPECIFIED Mandl, Claudia UNSPECIFIED UNSPECIFIED UNSPECIFIED Scholl, Catharina UNSPECIFIED UNSPECIFIED UNSPECIFIED Woelfl, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Winkler, Johannes UNSPECIFIED UNSPECIFIED UNSPECIFIED Gaspar, John A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Sachinidis, Agapios UNSPECIFIED UNSPECIFIED UNSPECIFIED Ciccolini, Francesca UNSPECIFIED orcid.org/0000-0002-7276-7361 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-448297
DOI:	10.1038/srep03803
Journal or Publication Title:	Sci Rep
Volume:	4
Date:	2014
Publisher:	NATURE RESEARCH
Place of Publication:	BERLIN
ISSN:	2045-2322
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language AMPLIFYING PROGENITORS; VASCULAR NICHE; ADULT SVZ; ACTIVATION; EXPRESSION; ANTIBODY; PROTEIN; SURFACE; FATE Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/44829