Universität zu Köln

Weber, Stefanie, Weiser, Barbara, Kemal, Kimdar S., Burger, Harold, Ramirez, Christina M., Korn, Klaus ORCID: 0000-0003-1891-2107, Anastos, Kathryn, Kaul, Rupert ORCID: 0000-0002-1586-4093, Kovacs, Colin and Doerfler, Walter (2014). Epigenetic analysis of HIV-1 proviral genomes from infected individuals: Predominance of unmethylated CpG's. Virology, 449. S. 181 - 190. SAN DIEGO: ACADEMIC PRESS INC ELSEVIER SCIENCE. ISSN 0042-6822

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Abstract

Efforts to cure HIV-1 infections aim at eliminating proviral DNA. Integrated DNA from various viruses often becomes methylated de novo and transcriptionally inactivated. We therefore investigated CpG methylation profiles of 55 of 94 CpG's (58.5%) in HIV-1 proviral genomes including ten CpG's in each LTR and additional CpG's in portions of gag, env, nef, rev, and tat genes. We analyzed 33 DNA samples from PBMC's of 23 subjects representing a broad spectrum of HIV-1 disease. In 22 of 23 HIV-1-infected individuals, there were only unmethylated CpG's regardless of infection status. In one long term nonprogressor, however, methylation of proviral DNA varied between 0 and 75% over an 11-year period although the CD4+ counts remained stable. Hence levels of proviral DNA methylation can fluctuate. The preponderance of unmethylated CpG's suggests that proviral methylation is not a major factor in regulating HIV-1 proviral activity in PBMC's. Unmethylated CpG's may play a role in HIV-1 immunopathogenesis. (C) 2013 Elsevier Inc. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Weber, Stefanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Weiser, Barbara UNSPECIFIED UNSPECIFIED UNSPECIFIED Kemal, Kimdar S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Burger, Harold UNSPECIFIED UNSPECIFIED UNSPECIFIED Ramirez, Christina M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Korn, Klaus UNSPECIFIED orcid.org/0000-0003-1891-2107 UNSPECIFIED Anastos, Kathryn UNSPECIFIED UNSPECIFIED UNSPECIFIED Kaul, Rupert UNSPECIFIED orcid.org/0000-0002-1586-4093 UNSPECIFIED Kovacs, Colin UNSPECIFIED UNSPECIFIED UNSPECIFIED Doerfler, Walter UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-448395
DOI:	10.1016/j.virol.2013.11.013
Journal or Publication Title:	Virology
Volume:	449
Page Range:	S. 181 - 190
Date:	2014
Publisher:	ACADEMIC PRESS INC ELSEVIER SCIENCE
Place of Publication:	SAN DIEGO
ISSN:	0042-6822
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HUMAN-IMMUNODEFICIENCY-VIRUS; ACTIVE ANTIRETROVIRAL THERAPY; DE-NOVO METHYLATION; DNA METHYLATION; LATENT RESERVOIR; HAMSTER-CELLS; FOREIGN DNA; T-CELLS; B-CELLS; EXPRESSION Multiple languages Virology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/44839