Wang, Lu, Cui, Yurong, Tang, Ming, Hu, Xinwu, Luo, Hongyan, Hescheler, Juergen and Xi, Jiaoya (2014). Puerarin Facilitates T-Tubule Development of Murine Embryonic Stem Cell-Derived Cardiomyocytes. Cell. Physiol. Biochem., 34 (2). S. 383 - 393. BASEL: KARGER. ISSN 1421-9778

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Abstract

Aims: The embryonic stem cell derived cardiomyocytes (ES-CM) is one of the promising cell sources for repopulation of damaged myocardium. However, ES-CMs present immature structure, which impairs their integration with host tissue and functional regeneration. This study used murine ES-CMs as an in vitro model of cardiomyogenesis to elucidate the effect of puerarin, the main compound found in the traditional Chinese medicine the herb Radix puerariae, on t-tubule development of murine ES-CMs. Methods: Electron microscope was employed to examine the ultrastructure. The investigation of transverse tubules (t-tubules) was performed by Di-8-ANEPPS staining. Quantitative real-time PCR was utilized to study the transcript level of genes related to t-tubule development. Results: We found that long-term application of puerarin throughout cardiac differentiation improved myofibril array and sarcomeres formation, and significantly facilitated t-tubules development of ES-CMs. The transcript levels of caveolin-3, amphiphysin-2 and junctophinlin-2, which are crucial for the formation and development of t-tubules, were significantly upregulated by puerarin treatment. Furthermore, puerarin repressed the expression of miR-22, which targets to caveolin-3. Conclusion: Our data showed that puerarin facilitates t-tubule development of murine ES-CMs. This might be related to the repression of miR-22 by puerarin and upregulation of Cav3, Bin1 and JP2 transcripts. Copyright (C) 2014 S. Karger AG, Basel

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Wang, LuUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Cui, YurongUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Tang, MingUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hu, XinwuUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Luo, HongyanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hescheler, JuergenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Xi, JiaoyaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-449428
DOI: 10.1159/000363007
Journal or Publication Title: Cell. Physiol. Biochem.
Volume: 34
Number: 2
Page Range: S. 383 - 393
Date: 2014
Publisher: KARGER
Place of Publication: BASEL
ISSN: 1421-9778
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
SARCOPLASMIC-RETICULUM; FUNCTIONAL-PROPERTIES; MATURATION; CONTRACTION; BIOGENESIS; CAVEOLAE; COMPLEX; ROLES; HEART; RATMultiple languages
Cell Biology; PhysiologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/44942

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