Universität zu Köln

Stuermer, Rene, Mueller, Stefan, Hanisch, Franz-Georg and Hoffmann, Werner ORCID: 0000-0003-0031-9540 (2014). Porcine Gastric TFF2 is a Mucus Constituent and Differs from Pancreatic TFF2. Cell. Physiol. Biochem., 33 (4). S. 895 - 905. BASEL: KARGER. ISSN 1421-9778

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Abstract

Background/Aims: TFF2 is a secretory peptide (106 amino acid residues) of the gastric mucosa, the porcine exocrine pancreas as well as immune cells and the CNS. It was the aim of this study to compare gastric and pancreatic TFF2. Methods: TFF2 was purified from the porcine stomach and pancreas, respectively, by size-exclusion and anion-exchange chromatography and then analyzed by Western blots as well as mass spectrometry. Results: Gastric and pancreatic TFF2 differed markedly, i.e. gastric TFF2 was exclusively associated with the high molecular mass mucus fraction, whereas pancreatic TFF2 appeared as a low molecular mass product. Unexpectedly, the latter predominantly formed a non-covalently linked homodimer resistant to boiling SDS. In contrast, gastric TFF2 is an integral mucus constituent predominantly binding to the mucin MUC6 in a non-covalent fashion. Conclusion: The non-covalent interaction of TFF2 with the mucin MUC6 is typical of a link peptide which is perfectly suited to assemble and stabilize the laminated structure of gastric mucus and to modulate its rheological properties. Copyright (C) 2014 S. Karger AG, Basel

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Stuermer, Rene UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueller, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Hanisch, Franz-Georg UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoffmann, Werner UNSPECIFIED orcid.org/0000-0003-0031-9540 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-450277
DOI:	10.1159/000358662
Journal or Publication Title:	Cell. Physiol. Biochem.
Volume:	33
Number:	4
Page Range:	S. 895 - 905
Date:	2014
Publisher:	KARGER
Place of Publication:	BASEL
ISSN:	1421-9778
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HUMAN SPASMOLYTIC POLYPEPTIDE; MUCOUS CELL MUCIN; TREFOIL PEPTIDES; HELICOBACTER-PYLORI; GROWTH-FACTOR; IN-VIVO; EXPRESSION; FAMILY; COLOCALIZATION; FACTOR-2 Multiple languages Cell Biology; Physiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/45027