Universität zu Köln

Siebelmann, Sebastian, Gehlsen, Uta ORCID: 0000-0001-9057-9199, Huettmann, Gereon, Koop, Norbert, Boelke, Torsten, Gebert, Andreas, Stern, Michael E., Niederkorn, Jerry Y. and Steven, Philipp (2013). Development, Alteration and Real Time Dynamics of Conjunctiva-Associated Lymphoid Tissue. PLoS One, 8 (12). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1932-6203

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Abstract

Purpose: Conjunctiva-associated lymphoid tissue (CALT) is thought to play a key role in initiating ocular surface related immune responses. This study was planned to get first profound insights into the function of CALT related to development, cellular dynamics and morphological alteration using a novel mouse model. Methods: Expression and morphology of CALT were investigated using BALB/c mice kept under different housing conditions, after topical antigen-stimulation and following lymphadenectomy and splenectomy. Particles and bacteria were applied topically to study antigen-transport. Intravital visualization was performed using two-photon microscopy. Results: Postnatal development and ultrastructure of CALT in the mouse is similar to humans. Topical antigen-challenge significantly alters CALT expression. Bacterial translocation is demonstrated via lymphoepithelium whereas cellular velocities within follicles were approximately 8 mu m/min. Conclusions: CALT in the mouse is an immunological interface of the ocular surface, featuring dynamic processes such as morphological plasticity, particle/bacteria transport and cellular migration.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Siebelmann, Sebastian UNSPECIFIED UNSPECIFIED UNSPECIFIED Gehlsen, Uta UNSPECIFIED orcid.org/0000-0001-9057-9199 UNSPECIFIED Huettmann, Gereon UNSPECIFIED UNSPECIFIED UNSPECIFIED Koop, Norbert UNSPECIFIED UNSPECIFIED UNSPECIFIED Boelke, Torsten UNSPECIFIED UNSPECIFIED UNSPECIFIED Gebert, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Stern, Michael E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Niederkorn, Jerry Y. UNSPECIFIED UNSPECIFIED UNSPECIFIED Steven, Philipp UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-469944
DOI:	10.1371/journal.pone.0082355
Journal or Publication Title:	PLoS One
Volume:	8
Number:	12
Date:	2013
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1932-6203
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language M-CELLS; T-CELLS; ANTIGEN; ACTIVATION; FOLLICLE; MUCOSA; INDUCTION; RABBIT; ROUTE; BIND Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/46994