Universität zu Köln

Ilyas, Shaista, Ilyas, Muhammad, van der Hoorn, Renier A. L. and Mathur, Sanjay (2013). Selective Conjugation of Proteins by Mining Active Proteomes through Click-Functionalized Magnetic Nanoparticles. ACS Nano, 7 (11). S. 9655 - 9664. WASHINGTON: AMER CHEMICAL SOC. ISSN 1936-086X

Full text not available from this repository.

Abstract

Superparamagnetic iron oxide nanoparticles (SPIONs) coated with azide groups were functionalized at the surface with biotin (biotin@SPIONs) and cysteine protease inhibitor E-64 (E-64@SPION5) with the purpose of developing nanoparticle-based assays for identifying cysteine proteases in proteomes. Magnetite particles (ca. 6 nm) were synthesized by microwave-assisted thermal decomposition of iron acetylacetonate and subsequently functionalized following a click chemistry protocol to obtain biotin and E-64 labeled particulate systems. Successful surface modification and covalent attachment of functional groups and molecules were confirmed by FT-IR spectroscopy and thermal gravimetric analysis. The ability of the surface-grafted biotin terminal groups to specifically interact with streptavidin (either horseradish peroxidase [(HRP)-luminol-H2O2] or rhodamine) was confirmed by chemiluminescent assay. A quantitative assessment showed a capture limit of 0.55-1.65 mu g protein/100 mu g particles. Furthermore, E-64@SPION5 were successfully used to specifically label papain-like cysteine proteases from crude plant extracts. Owing to the simplicity and versatility of the technique, together with the superparamagnetic behavior of FeOx-nanoparticles, the results demonstrate that click chemistry on surface anchored azide group is a viable approach toward bioconjugations that can be extended to other nanoparticles surfaces with different functional groups to target specific therapeutic and diagnostic applications.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Ilyas, Shaista UNSPECIFIED UNSPECIFIED UNSPECIFIED Ilyas, Muhammad UNSPECIFIED UNSPECIFIED UNSPECIFIED van der Hoorn, Renier A. L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mathur, Sanjay UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-472443
DOI:	10.1021/nn402382g
Journal or Publication Title:	ACS Nano
Volume:	7
Number:	11
Page Range:	S. 9655 - 9664
Date:	2013
Publisher:	AMER CHEMICAL SOC
Place of Publication:	WASHINGTON
ISSN:	1936-086X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language IRON-OXIDE NANOPARTICLES; BIOMEDICAL APPLICATIONS; CYSTEINE PROTEASES; CONTRAST AGENTS; IN-VITRO; CANCER; VERSATILE; SURFACES; PLATFORM; RELEASE Multiple languages Chemistry, Multidisciplinary; Chemistry, Physical; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/47244