Fusca, Debora, Husch, Andreas, Baumann, Arnd ORCID: 0000-0001-9456-7275 and Kloppenburg, Peter (2013). Choline acetyltransferase-like immunoreactivity in a physiologically distinct subtype of olfactory nonspiking local interneurons in the cockroach (periplaneta americana). J. Comp. Neurol., 521 (15). S. 3556 - 3570. HOBOKEN: WILEY-BLACKWELL. ISSN 1096-9861

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Abstract

Behavioral and physiological studies have shown that local interneurons are pivotal for processing odor information in the insect antennal lobe. They mediate inhibitory and excitatory interactions between the glomerular pathways and ultimately shape the tuning profile of projection neurons. To identify putative cholinergic local interneurons in the antennal lobe of Periplaneta americana, an antibody raised against the biosynthetic enzyme choline acetyltransferase (ChAT) was applied to individual morphologically and electrophysiologically characterized local interneurons. In nonspiking type IIa1 local interneurons, which were classified in this study, we found ChAT-like immunoreactivity suggesting that they are most likely excitatory. This is a well-defined population of neurons that generates Ca2+-driven spikelets upon depolarization and stimulation with odorants, but not Na+-driven action potentials, because they lack voltage-activated transient Na+ currents. The nonspiking type IIa2 and type IIb local interneurons, in which Ca2+-driven spikelets were absent, had no ChAT-like immunoreactivity. The GABA-like immunoreactive, spiking type I local interneurons had no ChAT-like immunoreactivity. In addition, we showed that uniglomerular projection neurons with cell bodies located in the ventral portion of the ventrolateral somata group and projections along the inner antennocerebral tract exhibited ChAT-like immunoreactivity. Assigning potential transmitters and neuromodulators to distinct morphological and electrophysiological types of antennal lobe neurons is an important prerequisite for a detailed understanding of odor information processing in insects. J. Comp. Neurol. 521:3556-3569, 2013. (c) 2013 Wiley Periodicals, Inc.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Fusca, DeboraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Husch, AndreasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Baumann, ArndUNSPECIFIEDorcid.org/0000-0001-9456-7275UNSPECIFIED
Kloppenburg, PeterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-475598
DOI: 10.1002/cne.23371
Journal or Publication Title: J. Comp. Neurol.
Volume: 521
Number: 15
Page Range: S. 3556 - 3570
Date: 2013
Publisher: WILEY-BLACKWELL
Place of Publication: HOBOKEN
ISSN: 1096-9861
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
DROSOPHILA ANTENNAL LOBE; MOTH MANDUCA-SEXTA; UNIGLOMERULAR PROJECTION NEURONS; GABA-LIKE IMMUNOREACTIVITY; SYNAPTIC CONNECTIONS; SPHINX MOTH; NEUROPEPTIDE EXPRESSION; NEUROBLAST LINEAGE; ODOR RESPONSES; INSECT BRAINMultiple languages
Neurosciences; ZoologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/47559

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