Universität zu Köln

Jaros, Julian A. J., Guest, Paul C., Ramoune, Hassan, Rothermundt, Matthias, Leweke, F. Markus ORCID: 0000-0002-8163-195X, Martins-de-Souza, Daniel ORCID: 0000-0003-3595-5846 and Bahn, Sabine (2012). Clinical use of phosphorylated proteins in blood serum analysed by immobilised metal ion affinity chromatography and mass spectrometry. J. Proteomics, 76. S. 36 - 43. AMSTERDAM: ELSEVIER. ISSN 1876-7737

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Abstract

The process of protein phosphorylation in cells is well studied in the context of a wide range of biologic functions such as signalling, cell cycle, cell growth and differentiation, and others. In contrast, little progress has been made in the investigation of protein phosphorylation specifically in blood. Here, we focussed on the phosphoproteome in human blood serum to study its extent and characteristics, and to explore the potential clinical utility. Immobilised metal ion affinity chromatography (IMAC) for the enrichment of intact phosphorylated proteins and label-free liquid chromatography mass spectrometry (LC-MSE) were used for the molecular analysis of a large number of serum samples. To obtain high-confidence results, phosphorylated peptides had to be detected in at least 2 out of 3 technical replicates per sample and in >70% of the serum samples drawn from 80 volunteers. Individual analysis of these 80 non-pooled samples resulted in the detection of 5825 unique phosphorylated peptides after filtering, which corresponded to 502 unique proteins. The results provided evidence that blood serum may be an untapped source of phosphoproteins suitable for potential use in understanding disease pathophysiology and for identification of disease and drug response biomarkers. This article is part of a Special Issue entitled: Integrated omics. (C) 2012 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Jaros, Julian A. J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Guest, Paul C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ramoune, Hassan UNSPECIFIED UNSPECIFIED UNSPECIFIED Rothermundt, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED Leweke, F. Markus UNSPECIFIED orcid.org/0000-0002-8163-195X UNSPECIFIED Martins-de-Souza, Daniel UNSPECIFIED orcid.org/0000-0003-3595-5846 UNSPECIFIED Bahn, Sabine UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-476899
DOI:	10.1016/j.jprot.2012.02.015
Journal or Publication Title:	J. Proteomics
Volume:	76
Page Range:	S. 36 - 43
Date:	2012
Publisher:	ELSEVIER
Place of Publication:	AMSTERDAM
ISSN:	1876-7737
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PLASMA-PROTEOME; EXPRESSION; PHOSPHOPROTEOME; IDENTIFICATION; BIOMARKERS; STABILITY; DISCOVERY; PRECURSOR; DEPLETION; TYROSINE Multiple languages Biochemical Research Methods Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/47689