Universität zu Köln

Scola, N., Wieland, U., Silling, S., Altmeyer, P., Stuecker, M. and Kreuter, A. (2012). Prevalence of human polyomaviruses in common and rare types of non-Merkel cell carcinoma skin cancer. Br. J. Dermatol., 167 (6). S. 1315 - 1321. HOBOKEN: WILEY. ISSN 1365-2133

Full text not available from this repository.

Abstract

Background Little is known about the association of human polyomaviruses (HPyVs) other than Merkel cell polyomavirus (MCPyV) with nonmelanoma skin cancer. Objectives To evaluate the presence of HPyV6, HPyV7, trichodysplasia spinulosa-associated polyomavirus (TSV), also called HPyV8, and the recently discovered HPyV9 in basal cell carcinoma (BCC), actinic keratosis (AK), squamous cell carcinoma in situ (SCCis), squamous cell carcinoma (SCC), keratoacanthoma (KA), microcystic adnexal carcinoma (MAC) and atypical fibroxanthoma (AFX). Methods Archival paraffin-embedded samples (n = 193: 41 BCC, 31 AK, 8 SCCis, 52 SCC, 42 KA, 5 MAC and 14 AFX) were analysed for the presence of the respective HPyV by polymerase chain reaction (PCR). HPyV DNA loads (HPyV DNA copies per beta-globin gene copy) were determined in all HPyV-positive samples by quantitative real-time PCR. Immunohistochemical analysis of MCPyV large T-antigen (LTA) expression was performed using the monoclonal antibody CM2B4. Results MCPyV DNA was found in 29% of BCC, 19% of AK, 25% of SCCis, 27% of SCC, 29% of KA, 0% of MAC and 29% of AFX. MCPyV DNA loads never exceeded 0.3 MCPyV DNA copies per beta-globin gene copy (median 0.004). In the immunohistochemical analysis of MCPyV LTA expression, all evaluated samples (32 MCPyV DNA-positive samples) were LTA negative. HPyV6 DNA was found in 7% of BCC, 3% of AK, 12% of SCCis, 4% of SCC, 5% of KA, and 0% of MAC and AFX. HPyV6 DNA loads never exceeded 0.7 HPyV6 DNA copies per beta-globin gene copy (median 0.015). None of the 193 samples was positive for HPyV7, TSV or HPyV9 DNA. Conclusions Our findings argue against a pathogenic role for MCPyV, HPyV6, HPyV7, TSV and HPyV9 in the analysed types of non-Merkel cell carcinoma skin cancer.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Scola, N. UNSPECIFIED UNSPECIFIED UNSPECIFIED Wieland, U. UNSPECIFIED UNSPECIFIED UNSPECIFIED Silling, S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Altmeyer, P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Stuecker, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kreuter, A. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-477669
DOI:	10.1111/j.1365-2133.2012.11141.x
Journal or Publication Title:	Br. J. Dermatol.
Volume:	167
Number:	6
Page Range:	S. 1315 - 1321
Date:	2012
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1365-2133
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DNA; IDENTIFICATION Multiple languages Dermatology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/47766