Peleg, Anton Y., de Breij, Anna, Adams, Mark D., Cerqueira, Gustavo M., Mocali, Stefano ORCID: 0000-0002-1173-7206, Galardini, Marco ORCID: 0000-0003-2018-8242, Nibbering, Peter H., Earl, Ashlee M., Ward, Doyle V., Paterson, David L., Seifert, Harald and Dijkshoorn, Lenie (2012). The Success of Acinetobacter Species; Genetic, Metabolic and Virulence Attributes. PLoS One, 7 (10). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1932-6203

Full text not available from this repository.

Abstract

An understanding of why certain Acinetobacter species are more successful in causing nosocomial infections, transmission and epidemic spread in healthcare institutions compared with other species is lacking. We used genomic, phenotypic and virulence studies to identify differences between Acinetobacter species. Fourteen strains representing nine species were examined. Genomic analysis of six strains showed that the A. baumannii core genome contains many genes important for diverse metabolism and survival in the host. Most of the A. baumannii core genes were also present in one or more of the less clinically successful species. In contrast, when the accessory genome of an individual A. baumannii strain was compared to a strain of a less successful species (A. calcoaceticus RUH2202), many operons with putative virulence function were found to be present only in the A. baumannii strain, including the csu operon, the acinetobactin chromosomal cluster, and bacterial defence mechanisms. Phenotype microarray analysis showed that compared to A. calcoaceticus (RUH2202), A. baumannii ATCC 19606(T) was able to utilise nitrogen sources more effectively and was more tolerant to pH, osmotic and antimicrobial stress. Virulence differences were also observed, with A. baumannii ATCC 19606(T), A. pittii SH024, and A. nosocomialis RUH2624 persisting and forming larger biofilms on human skin than A. calcoaceticus. A. baumannii ATCC 19606 T and A. pittii SH024 were also able to survive in a murine thigh infection model, whereas the other two species were eradicated. The current study provides important insights into the elucidation of differences in clinical relevance among Acinetobacter species.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Peleg, Anton Y.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
de Breij, AnnaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Adams, Mark D.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Cerqueira, Gustavo M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mocali, StefanoUNSPECIFIEDorcid.org/0000-0002-1173-7206UNSPECIFIED
Galardini, MarcoUNSPECIFIEDorcid.org/0000-0003-2018-8242UNSPECIFIED
Nibbering, Peter H.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Earl, Ashlee M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ward, Doyle V.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Paterson, David L.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Seifert, HaraldUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Dijkshoorn, LenieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-480846
DOI: 10.1371/journal.pone.0046984
Journal or Publication Title: PLoS One
Volume: 7
Number: 10
Date: 2012
Publisher: PUBLIC LIBRARY SCIENCE
Place of Publication: SAN FRANCISCO
ISSN: 1932-6203
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
HUMAN SKIN EQUIVALENT; MOLECULAR CHARACTERIZATION; BAUMANNII COMPLEX; BIOFILM FORMATION; GENOME SEQUENCE; IDENTIFICATION; SIDEROPHORE; ACQUISITION; EVOLUTION; ADHERENCEMultiple languages
Multidisciplinary SciencesMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/48084

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item