Universität zu Köln

Eklund, D. Magnus, Cierlik, Izabela, Staldal, Veronika, Claes, Andrea R., Vestman, Daniel, Chandler, John and Sundberg, Eva (2011). Expression of Arabidopsis SHORT INTERNODES/STYLISH Family Genes in Auxin Biosynthesis Zones of Aerial Organs Is Dependent on a GCC Box-Like Regulatory Element. Plant Physiol., 157 (4). S. 2069 - 2081. ROCKVILLE: AMER SOC PLANT BIOLOGISTS. ISSN 0032-0889

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Abstract

Auxin/indole-3-acetic acid (IAA) biosynthesis in Arabidopsis (Arabidopsis thaliana) plays a major role in growth responses to developmental and genetic signals as well as to environmental stimuli. Knowledge of its regulation, however, remains rudimentary, and few proteins acting as transcriptional modulators of auxin biosynthesis have been identified. We have previously shown that alteration in the expression level of the SHORT INTERNODES/STYLISH (SHI/STY) family member STY1 affects IAA biosynthesis rates and IAA levels and that STY1 acts as a transcriptional activator of genes encoding auxin biosynthesis enzymes. Here, we have analyzed the upstream regulation of SHI/STY family members to gain further insight into transcriptional regulation of auxin biosynthesis. We attempted to modulate the normal expression pattern of STY1 by mutating a putative regulatory element, a GCC box, located in the proximal promoter region and conserved in most SHI/STY genes in Arabidopsis. Mutations in the GCC box abolish expression in aerial organs of the adult plant. We also show that induction of the transcriptional activator DORNROSCHEN-LIKE (DRNL) activates the transcription of STY1 and other SHI/STY family members and that this activation is dependent on a functional GCC box. Additionally, STY1 expression in the strong drnl-2 mutant or the drn drnl-1 puchi-1 triple mutant, carrying knockdown mutations in both DRNL and its close paralogue DRN as well as one of their closest homologs, PUCHI, was significantly reduced, suggesting that DRNL regulates STY1 during normal plant development and that several other genes might have redundant functions.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Eklund, D. Magnus UNSPECIFIED UNSPECIFIED UNSPECIFIED Cierlik, Izabela UNSPECIFIED UNSPECIFIED UNSPECIFIED Staldal, Veronika UNSPECIFIED UNSPECIFIED UNSPECIFIED Claes, Andrea R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Vestman, Daniel UNSPECIFIED UNSPECIFIED UNSPECIFIED Chandler, John UNSPECIFIED UNSPECIFIED UNSPECIFIED Sundberg, Eva UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-483807
DOI:	10.1104/pp.111.182253
Journal or Publication Title:	Plant Physiol.
Volume:	157
Number:	4
Page Range:	S. 2069 - 2081
Date:	2011
Publisher:	AMER SOC PLANT BIOLOGISTS
Place of Publication:	ROCKVILLE
ISSN:	0032-0889
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language YUCCA FLAVIN MONOOXYGENASES; GIBBERELLIN RESPONSES; GYNOECIUM DEVELOPMENT; TRANSCRIPTION FACTOR; PLANT DEVELOPMENT; DORNROSCHEN-LIKE; PROTEIN; STY1; IDENTIFICATION; TISSUES Multiple languages Plant Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48380