Universität zu Köln

Bostanci, N., Akguel, B., Tsakanika, V., Allaker, R. P., Hughes, F. J. and McKay, I. J. (2011). Effects of low-dose doxycycline on cytokine secretion in human monocytes stimulated with Aggregatibacter actinomycetemcomitans. Cytokine, 56 (3). S. 656 - 662. LONDON: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD. ISSN 1043-4666

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Abstract

Doxycycline is an antibiotic used in the treatment of a variety of inflammatory conditions, including periodontitis. Apart from its antimicrobial properties, this drug also has independent anti-inflammatory effects at sub-antimicrobial doses. The present study aimed to investigate the effects of low-doses of doxycycline (LDD) on cytokine production by human monocytic cells challenged with the periodontal pathogen Aggregatibacter actinomycetemcomitans, for up to 6 h. The simultaneous regulation of 12 cytokines were measured by a Human Cytokine Array Kit. To validate the array findings, selected cytokines were also measured by enzyme-linked immunosorbant assay (ELISA). A. actinomycetemcomitans stimulated the production of tumor necrosis factor (TNF)-alpha, interleukin (IL)-1 alpha, IL-1 beta, IL-6 and IL-8 by the cells after 6 h of challenge, and doxycycline significantly inhibited this effect. The kinetics of this regulation demonstrated an early (within 2 h) and significant (P < 0.05) inhibition of pro-inflammatory cytokines, with a mild (0.5-fold) up-regulation of the anti-inflammatory cytokine IL-10. The results indicate that LDD acts as an anti-inflammatory agent in human monocytic cells stimulated with A. actinomycetemcomitans. This model provides clear evidence that some of the clinically proven benefits of LDD may be related to its ability to regulate inflammatory mediator release by monocytic cells. This property may contribute to the clinically proven benefits of this antibiotic as an adjunctive treatment for periodontitis. (C) 2011 Elsevier Ltd. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Bostanci, N. UNSPECIFIED UNSPECIFIED UNSPECIFIED Akguel, B. UNSPECIFIED UNSPECIFIED UNSPECIFIED Tsakanika, V. UNSPECIFIED UNSPECIFIED UNSPECIFIED Allaker, R. P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hughes, F. J. UNSPECIFIED UNSPECIFIED UNSPECIFIED McKay, I. J. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-483886
DOI:	10.1016/j.cyto.2011.08.039
Journal or Publication Title:	Cytokine
Volume:	56
Number:	3
Page Range:	S. 656 - 662
Date:	2011
Publisher:	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Place of Publication:	LONDON
ISSN:	1043-4666
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CYTOLETHAL DISTENDING TOXIN; TUMOR-NECROSIS-FACTOR; HUMAN GINGIVAL FIBROBLASTS; HUMAN WHOLE-BLOOD; ACTINOBACILLUS-ACTINOMYCETEMCOMITANS; PORPHYROMONAS-GINGIVALIS; CHRONIC PERIODONTITIS; CREVICULAR FLUID; JUVENILE PERIODONTITIS; GENE-EXPRESSION Multiple languages Biochemistry & Molecular Biology; Cell Biology; Immunology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48388