Universität zu Köln

Castaneda, Rosalinda T., Boddington, Sophie, Henning, Tobias D., Wendland, Mike, Mandrussow, Lydia, Liu, Siyuan and Daldrup-Link, Heike (2011). Labeling human embryonic stem-cell-derived cardiomyocytes for tracking with MR imaging. Pediatr. Radiol., 41 (11). S. 1384 - 1393. NEW YORK: SPRINGER. ISSN 1432-1998

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Abstract

Background Human embryonic stem cells (hESC) can generate cardiomyocytes (CM), which offer promising treatments for cardiomyopathies in children. However, challenges for clinical translation result from loss of transplanted cell from target sites and high cell death. An imaging technique that noninvasively and repetitively monitors transplanted hESC-CM could guide improvements in transplantation techniques and advance therapies. Objective To develop a clinically applicable labeling technique for hESC-CM with FDA-approved superparamagnetic iron oxide nanoparticles (SPIO) by examining labeling before and after CM differentiation. Materials and methods Triplicates of hESC were labeled by simple incubation with 50 mu g/ml of ferumoxides before or after differentiation into CM, then imaged on a 7T MR scanner using a T2-weighted multi-echo spin-echo sequence. Viability, iron uptake and T2-relaxation times were compared between groups using t-tests. Results hESC-CM labeled before differentiation demonstrated significant MR effects, iron uptake and preserved function. hESC-CM labeled after differentiation showed no significant iron uptake or change in MR signal (P < 0.05). Morphology, differentiation and viability were consistent between experimental groups. Conclusion hESC-CM should be labeled prior to CM differentiation to achieve a significant MR signal. This technique permits monitoring delivery and engraftment of hESC-CM for potential advancements of stem cell-based therapies in the reconstitution of damaged myocardium.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Castaneda, Rosalinda T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Boddington, Sophie UNSPECIFIED UNSPECIFIED UNSPECIFIED Henning, Tobias D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Wendland, Mike UNSPECIFIED UNSPECIFIED UNSPECIFIED Mandrussow, Lydia UNSPECIFIED UNSPECIFIED UNSPECIFIED Liu, Siyuan UNSPECIFIED UNSPECIFIED UNSPECIFIED Daldrup-Link, Heike UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-486797
DOI:	10.1007/s00247-011-2130-3
Journal or Publication Title:	Pediatr. Radiol.
Volume:	41
Number:	11
Page Range:	S. 1384 - 1393
Date:	2011
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1432-1998
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language POSITRON-EMISSION-TOMOGRAPHY; IN-VIVO; CONTRAST AGENTS; MYOCARDIAL-INFARCTION; BONE-MARROW; PROTAMINE SULFATE; INDOCYANINE GREEN; STROMAL CELLS; FERUMOXIDES; DELIVERY Multiple languages Pediatrics; Radiology, Nuclear Medicine & Medical Imaging Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48679