Universität zu Köln

Kretschmer, Celine, Conradi, Anne, Kemmner, Wolfgang and Sterner-Kock, Anja (2011). Latent transforming growth factor binding protein 4 (LTBP4) is downregulated in mouse and human DCIS and mammary carcinomas. Cell. Oncol., 34 (5). S. 419 - 435. DORDRECHT: SPRINGER. ISSN 2211-3436

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Abstract

Transforming growth factor beta (TGF-) is able to inhibit the proliferation of epithelial cells and is involved in the carcinogenesis of mammary tumors. Three latent transforming growth factor- binding proteins (LTBPs) are known to modulate TGF- functions. The current study analyses the expression profiles of LTBP4, its isoforms LTBP1 and LTBP3, and TGF-1, TGF-2, TGF-3, and SMAD2, SMAD3 and SMAD4 in human and murine (WAP-TNP8) DCIS compared to invasive mammary tumors. Additionally mammary malignant (MCF7, Hs578T, MDA-MB361) and non malignant cell lines (Hs578BsT) were analysed. Microarray, q-PCR, immunoblot, immunohistochemistry and immunofluorescence were used. In comparison to non-malignant tissues (n = 5), LTBP4 was downregulated in all human and mouse DCIS (n = 9) and invasive mammary adenocarcinomas (n = 5) that were investigated. We also found decreased expression of bone morphogenic protein 4 (BMP4) and increased expression of its inhibitor gremlin (GREM1). Treatment of the mammary tumor cell line (Hs578T) with recombinant TGF-1 rescued BMP4 and GREM1 expression. We conclude that the lack of LTBP4-mediated targeting in malignant mammary tumor tissues may lead to a possible modification of TGF-1 and BMP bioavailability and function.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kretschmer, Celine UNSPECIFIED UNSPECIFIED UNSPECIFIED Conradi, Anne UNSPECIFIED UNSPECIFIED UNSPECIFIED Kemmner, Wolfgang UNSPECIFIED UNSPECIFIED UNSPECIFIED Sterner-Kock, Anja UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-487643
DOI:	10.1007/s13402-011-0023-y
Journal or Publication Title:	Cell. Oncol.
Volume:	34
Number:	5
Page Range:	S. 419 - 435
Date:	2011
Publisher:	SPRINGER
Place of Publication:	DORDRECHT
ISSN:	2211-3436
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language BREAST-CANCER CELLS; FIBROBLAST EXTRACELLULAR-MATRIX; BONE MORPHOGENETIC PROTEINS; GENE-EXPRESSION PROFILES; TGF-BETA; MESSENGER-RNA; IN-SITU; BINDING-PROTEIN-4 LTBP-4; MOLECULAR-CLONING; EPITHELIAL-CELLS Multiple languages Oncology; Cell Biology; Pathology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48764