Universität zu Köln

Kohler, Maxie, Schaenzer, Wilhelm and Thevis, Mario (2011). RNA interference for performance enhancement and detection in doping control. Drug Test. Anal., 3 (10). S. 661 - 668. MALDEN: WILEY-BLACKWELL. ISSN 1942-7603

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Abstract

RNA interference represents a comparably new route of regulating and manipulating specific gene expression. Promising results were obtained in experimental therapies aim at the treatment of different kinds of diseases including cancer, diabetes mellitus or Dychenne muscular dystrophy. While studies on down-regulation efficiency are often performed by analyzing the regulated protein, the direct detection of small, interfering RNA molecules and antisense oligonucleotides is of great interest for the investigation of the metabolism and degradation and also for the detection of a putative misuse of these molecules in sports. Myostatin down-regulation was shown to result in increased performance and muscle growth and the regulation of several other proteins could be relevant for performance enhancement. This mini-review summarizes current approaches for the mass spectrometric analysis of siRNA and antisense oligonucleotides from biological matrices and the available data on biodistribution, metabolism, and half-life of relevant substances are discussed. Copyright (C) 2011 John Wiley & Sons, Ltd.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kohler, Maxie UNSPECIFIED UNSPECIFIED UNSPECIFIED Schaenzer, Wilhelm UNSPECIFIED UNSPECIFIED UNSPECIFIED Thevis, Mario UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-487760
DOI:	10.1002/dta.330
Journal or Publication Title:	Drug Test. Anal.
Volume:	3
Number:	10
Page Range:	S. 661 - 668
Date:	2011
Publisher:	WILEY-BLACKWELL
Place of Publication:	MALDEN
ISSN:	1942-7603
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MASS-SPECTROMETRY; CHEMICAL-MODIFICATION; ANTISENSE OLIGONUCLEOTIDE; THERAPEUTIC APPLICATIONS; MUSCLE HYPERTROPHY; IN-VIVO; STABILITY; SIRNA; DUPLEX; MICE Multiple languages Biochemical Research Methods; Chemistry, Analytical; Pharmacology & Pharmacy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48776