Universität zu Köln

Uhrmacher, Sabrina, Schmidt, Christina, Erdfelder, Felix, Poll-Wolbeck, Simon Jonas, Gehrke, Iris, Hallek, Michael and Kreuzer, Karl-Anton (2011). Use of the receptor tyrosine kinase-like orphan receptor 1 (ROR1) as a diagnostic tool in chronic lymphocytic leukemia (CLL). Leuk. Res., 35 (10). S. 1360 - 1367. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD. ISSN 1873-5835

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Abstract

Flow cytometry is commonly used to establish the diagnosis of chronic lymphocytic leukemia (CLL). A defined combination of antibodies discriminates between normal B cells and CLL cells (coexpression of CD5, CD19, and CD23). The receptor tyrosine-like orphan receptor one (ROR1) is an embryonic glycoprotein involved in several developmental processes. It was shown to be highly and specifically expressed on circulating B lymphoma cells, but not on normal B cells. Here, we examined the potential of ROR1 as a diagnostic marker in initial and follow-up diagnostics of patients with CLL. 105 untreated and 72 treated patients, as well as healthy volunteers were examined using flow cytometry assays. Furthermore, we examined 10 patients with various B cell non-Hodgkin lymphomas (B-NHL). ROR1 was detected using a directly labeled antibody. We detected uniformly high ROR1 expression levels in all CLL samples. In marked contrast, only low or absent ROR1 expression levels were found on B cells from healthy donors. ROR1 expression in CLL patients was not influenced by various treatments. Taken together, ROR1 may be used as a diagnostic marker for CLL. As it is the only antigen which can exclusively be detected on neoplastic B cells it may greatly increase both, specificity as well as sensitivity, in lymphoma diagnostics. (C) 2011 Elsevier Ltd. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Uhrmacher, Sabrina UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmidt, Christina UNSPECIFIED UNSPECIFIED UNSPECIFIED Erdfelder, Felix UNSPECIFIED UNSPECIFIED UNSPECIFIED Poll-Wolbeck, Simon Jonas UNSPECIFIED UNSPECIFIED UNSPECIFIED Gehrke, Iris UNSPECIFIED UNSPECIFIED UNSPECIFIED Hallek, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Kreuzer, Karl-Anton UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-488720
DOI:	10.1016/j.leukres.2011.04.006
Journal or Publication Title:	Leuk. Res.
Volume:	35
Number:	10
Page Range:	S. 1360 - 1367
Date:	2011
Publisher:	PERGAMON-ELSEVIER SCIENCE LTD
Place of Publication:	OXFORD
ISSN:	1873-5835
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EXPRESSION; FLOW; MUTATIONS; ZAP-70; PCR Multiple languages Oncology; Hematology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48872