Wolf, Sylvi, Rataj, Felicitas, Zierau, Oliver, Ostermann, Kai, Diel, Patrick, Parr, Maria Kristina ORCID: 0000-0001-7407-8300, Vollmer, Guenter and Roedel, Gerhard (2010). A novel combined approach to detect androgenic activities with yeast based assays in Schizosaccharomyces pombe and Saccharomyces cerevisiae. Toxicol. Lett., 199 (3). S. 410 - 416. CLARE: ELSEVIER IRELAND LTD. ISSN 1879-3169

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Abstract

We describe the construction and validation of novel test systems for detecting androgenic activities using a combination of the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe. By applying the reporter enhanced Green Fluorescent Protein (EGFP) the incubation time could be reduced to only 24 h if compared to the classical p-galactosidase reporter (48 h). Both yeast systems were validated by analyzing the effects of seven androgens as well as five anti-androgens. One androgen (stanozolol) could be detected ten times more sensitive in S. cerevisiae than in S. pombe. Three of the five anti-androgenic substances showed no or only a slight effect in both yeast assays. The other two anti-androgens could be detected much better in S. pombe. Additionally, we could show that both yeast assays tolerated 10% urine within the media and still were capable to detect dihydrotestosterone at a concentration of 10(-8) M suggesting the use of the assays for applied doping pre-screening. In summary, the novel androgen-sensitive yeast assays have a large potential for various applications, e.g. as pre-screening in doping analysis or cattle feeding. A combination of both assays, exploiting these two phylogenetic very different yeasts, allows detection of the activity of a wide range of androgenic substances. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Wolf, SylviUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rataj, FelicitasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Zierau, OliverUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ostermann, KaiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Diel, PatrickUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Parr, Maria KristinaUNSPECIFIEDorcid.org/0000-0001-7407-8300UNSPECIFIED
Vollmer, GuenterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Roedel, GerhardUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-490639
DOI: 10.1016/j.toxlet.2010.10.007
Journal or Publication Title: Toxicol. Lett.
Volume: 199
Number: 3
Page Range: S. 410 - 416
Date: 2010
Publisher: ELSEVIER IRELAND LTD
Place of Publication: CLARE
ISSN: 1879-3169
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
HUMAN CELL-LINE; FISSION YEAST; RECEPTOR; EXPRESSION; REPORTER; VECTORSMultiple languages
ToxicologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/49063

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